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What will be the voltage you have to use to run agarose gel electrophoresis for the best resolution of DNA fragments if the distance between the two electrodes is around 15cm?
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∙ 11y ago
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∙ 11y ago
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What is meant by limit of resolution?
It is defined as the capacity of an optical system to resolve point objects as separate objects. Larger the aperture of optical system greater will be its limits of resolution.
What kind of rock indicates the short-distance transport of sediment?
The sedimentary rock breccia indicates short distance transport of sediments because the rock fragments that make it up are still angular and not rounded from abrasion.
What does resolving power of a microscope depend on?
In a light microscope the resolution of the image it can project is limited by the distance each photon travels in its wavelength. Beneath this minimum distance, the "noise" of the photon's movement along its path overwhelms any resolution the light source may otherwise provide.
What technique is used to improve the resolution of radio telescopes?
Base-line Interferometry is one way, done by increasing the distance between 'bases'.
In a gel electrophoresis's why are the lines closer together when the DNA strands are heavier?
Because the DNA cannot travel very long and spread apart when the strands are heavy. As a result, the DNA strands will all travel a relatively short distance and be closer together.
Why do you need a molecular weight ruler alongside your samples during electrophoresis?
A molecular weight ruler uses a sample of fragments of a known size (known as a molecular weight marker) to be placed alongside the experimental and control samples. It helps compare the migration distance of the experimental fragments to the migrating distance of the fragments of a known size that make up the molecular weight marker. Then the scientist can calculate an approx. size of their experimental samples.
Does the distance between electrodes affect the voltage?
Not materially. The voltage is determined by the composition of the electrodes and the medium in which they are placed.
What distance can 75 pounds of explosives propel fragments?
1 mile
What are the uses of electrophoresis?
Electrophoresis is a technique used for the separation of biological molecules based on their movement due to the influence of a direct electric current. The technique was pioneered in 1937 by the Swedish chemist Arne Tiselius for the separation of proteins. It has now been extended to the separation of many other different classes of biomolecules including nucleic acids, carbohydrates and amino acids. Electrophoresis has become increasingly important in the laboratory for basic research, biomedical research and in clinical settings for the diagnosis of disease. Electrophoresis is not commonly used to purify proteins in large quantities because other methods exist which are simpler, faster, and more efficient. However, it is valuable as an analytical technique for detecting and quantifying minute traces of many biomolecules in a mixture. It is also useful for determining certain physical properties such as molecular mass, isoelectric point, and biological activity.
How 75 pounds of explosive can propel fragments up to what distance?
1 mile
What distance can 75 pounds of fragment explosives propel fragments?
1 mile
Function of agarose in agarose gel electrophoresis?
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
The minimum distance between two object before they are seen as one object is known as?
resolution
What is spark plug gap of a 2000 mystique?
the distance between two electrodes measure in thousandth of a inche .054 on a mystique
Does increase in distance of separation between electrode increases conductivity of an electrolyte?
By definition, the conductivity of an electrolyte is going to be a constant value dependendent on a specific distance between electrodes, when measured. If you allow the distance between electrodes to vary, thus changing the definition of Conductivity, then the value of whatever you call this 'new thing' will DECREASE, as the distance increases, while all else remains equal.Suppose it takes 60 volts AC to create a 1 amp current flow through the electrolyte with the electrodes 1 inch apart. Then you move the electrodes 2 inches apart and it takes 120 volts AC to get the same 1 amp current flow. The farther apart the electrodes are, the greater the voltage required to recreate the same 1 amp current flow. The electrolyte has not changed physically or chemically, so its actual Conductivity has not changed, but that 'new thing' you defined above has changed.
What is the definition of resolution in science?
Resolution is the minimum distance between two objects. However, the two objects can still be viewed and observed as separate entities.
