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What will happen if the sample spots are under the surface level of the solvent?
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What would happen if the Spot of origin is lowered into the solvent in chromatography?
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!
Why is it important that the sample spots are not below the solvent level when the filter paper is first placed in the developing chamber?
By placing the spot above the level of the solvent in the beaker, you slow the flow of solvent which results in improved separation of the components in the spot. If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away.
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
What are the advantages and disadvantages of solvent extraction?
The advantages are: - Goldfish requires less solvent than Soxhlet (solvent in Soxhlet has to reach the siphon level). - Goldfish is quicker because the sample is constantly in contact with the solvent. Source: CECCHI, Heloísa Máscia."Fundamentos Teóricos e Práticos em Análise de Alimentos". Editora Unicamp. 2a Edição. 207p. 2003.
Why is it important to mark the solvent level on the paper immediately upon removing it?
To get an accurate measurement. Solvent will evporate.
What would happen if the Spot of origin is lowered into the solvent in chromatography?
If the original spots were below the level of developing solvent then the components of the spotted sample would dissolve into the solvent and no spots would be seen upon analysis because all of the sample is dissolved in the developing solvent. hope this helps. good luck in o. chem!
Why is it important that the sample spots are not below the solvent level when the filter paper is first placed in the developing chamber?
By placing the spot above the level of the solvent in the beaker, you slow the flow of solvent which results in improved separation of the components in the spot. If you placed it below the level of the solvent in the beaker, the solvent would just flow at a much faster rate and instead of separating the components in the spot, they would simply be washed away.
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
What would happen if you submerge the chromatography paper above the pencil line?
Try and see: if it wouldn't be above the level of solvent, the spots will run off.
What Suppose a bicycle was coasting on a level surface and there was no friction. What would happen to the bicycle's speed?
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What are the advantages and disadvantages of solvent extraction?
The advantages are: - Goldfish requires less solvent than Soxhlet (solvent in Soxhlet has to reach the siphon level). - Goldfish is quicker because the sample is constantly in contact with the solvent. Source: CECCHI, Heloísa Máscia."Fundamentos Teóricos e Práticos em Análise de Alimentos". Editora Unicamp. 2a Edição. 207p. 2003.
Why must the level ofthe solvent not reach the pencil line where the spots are?
if it wouldn't be above the level of solvent the spots will run off
Why is it important to mark the solvent level on the paper immediately upon removing it?
To get an accurate measurement. Solvent will evporate.
What is the surface-level of a movie?
What is the surface-level subject in a film?
How does sample size affect your level of confidence in accepting a hypothesis?
The larger the sample size the more confident you can be that the data you have collected is representative of what would happen on a larger scale. So if your results seem to prove your hypothesis right then the larger you sample size the more confident you can be in accepting your hypothesis.
A flat level surface?
A flat, level surface is a plane.
What are the elements that affect determination in a sample size?
Margin of error, level of significance and level of power are all elements that will affect the determination of sample size.
