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Q: When a fixed smear of bacterial cells come in contact with methylene blue and all cells appear blue under the oil lens this is an example of?
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Why is methylene blue is an appropriate dye for staining bacterial cells?

It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.


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your sample can destroy the bacteria and it will appear shapeless or enlarged when you overheat the bacterial smear


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