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What is the role of DMSO in cell culture?
It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
Why is DMSO used in PCR?
reduce secondary structure
Which solvent can dissolve grape seed oil?
dmso
Is glycerol a discaccharide?
No, glycerol is subunit of lipids.
Which is NOT a smaller subunit of a nucleotide?
Glycerol is not a subunit of nucleotides. Glycerol is a subunit of triglycerides and phospholipids (types of lipids).
What is the geometry of Ph3SnCl and DMSO complex?
Use the link below to begin your investigation of the geometry of Ph3SnCl and the polar aprotic solvent DMSO (dimethyl sulfoxide).
What are types of cryoprotectant?
1. DMSO 2. Ethylene glycol 3. Glycerol 4. 2-Methyl-2,4-pentanediol 5. Propylene glycol 6. Sucrose 7. Methylcellosolve 8. Dimethylformamide
How do you use glycerol in a sentence?
Medicine made by mixing a substance is glycerol
What is the difference betwen DMSO and DMSO-d6 solution?
One difference is that DMSO-d6 is going to cost more. Chemically speaking, the diffference is that the hydrogens in the -d6 variety have been replaced by deuterium.
Why is DMSO used as a control?
Many test compounds (drugs, inhibitors, etc) are not soluble in water and therefore dimethylsulfoxide (DMSO) is used as a solvent instead. The compound dissolved in DMSO is what is used to treat the cell or animal and therefore you must prove that it is the compound, not the DMSO which is causing any results seen. To ensure this, you have a control that contains only DMSO and not the test compound. This is often referred to as a "vehicle control".
How do you make large clear DMSO crystals?
DMSO (dimetylsulf oxide) (CH3)SO is a liquid with the freezing point at 19 oC.
Why use glycerol in competent cell preparation?
Glycerol is a cryo preservative gives density for samples to settle in well
What is the role of DMSO in cell culture?
It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
Why PCR need gelatin?
According to Roche website, different additives allow optimization to increase yield and specificity of PCR reactions. DMSO, for instance, is reported to reduce nonspecific priming, while gelatin and glycerol stabilize Taq DNA polymerase during PCR, which generally increases the yield.
Why DMSO does not need sterilization?
because microorganism will not grow in it
Why is DMSO used in PCR?
reduce secondary structure
Which solvent can dissolve grape seed oil?
dmso
