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reduce secondary structure
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What is the role of DMSO in cell culture?
It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
Which is used to copy DNA for DNA fingerprinting?
PCR
What Cryoprotectants used for embryonic stem cells?
Type your answer here..DMSO V used for cyropreservation..........
What equipments is used for collection and or detection of biological agents?
PCR
Why PCR need gelatin?
According to Roche website, different additives allow optimization to increase yield and specificity of PCR reactions. DMSO, for instance, is reported to reduce nonspecific priming, while gelatin and glycerol stabilize Taq DNA polymerase during PCR, which generally increases the yield.
Why is DMSO used as a control?
Many test compounds (drugs, inhibitors, etc) are not soluble in water and therefore dimethylsulfoxide (DMSO) is used as a solvent instead. The compound dissolved in DMSO is what is used to treat the cell or animal and therefore you must prove that it is the compound, not the DMSO which is causing any results seen. To ensure this, you have a control that contains only DMSO and not the test compound. This is often referred to as a "vehicle control".
What is the role of DMSO in cell culture?
It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
Which is used to copy DNA for DNA fingerprinting?
PCR
What Cryoprotectants used for embryonic stem cells?
Type your answer here..DMSO V used for cyropreservation..........
How do you dilute cyclosporine?
In organic solvents (DMSO, ethanol, chloroform, methylene chloride). The most common solvent used to dissolve cyclosporine is DMSO. Water will not work very well.
What is the purpose of the mineral oil used for PCR when the termocycler does not have a hot lid?
To prevent evaporation of PCR products.
What is quantitative pcr technology used for?
Quantitative PCR Technology is used in biochemistry, in particular molecular biology. The PCR stands for polymerase chain reaction and is used to "amplify" pieces of DNA to make millions of copies of a particular DNA strand.
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is the defference between Real-time PCR and reverse transcriptase PCR?
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
Role of magnesium chloride in PCR?
mgcl2 acts as a cofactor for the DNA polymerase used and is necessary for efficient functioning of the pcr
