An inoculating loop has a small circular ring at its tip and is used to collect cultures that are based in liquid. On the other hand, an inoculating needle can extract microorganisms from solid or dense material so they can be transferred to another location like a petri dish.
a loop will make the surface area a lot bigger for microorganisms to attach to, in comparison with a needle's point. also, needled tend to destroy the surface of the agar and may make the microorganism colony structure uneven for characterization.
An inoculating needle is used on solid media because the culture is more dense as well as the fact that the smaller tip of the needle allows you to pick up bacteria from a single CFU (colony forming unit) while avoiding contamination from other colonies on the same solid media. With liquid media, there is no distinction between the different colonies so a loop can be used, as well as the fact that it is easier to pick up liquid with a loop than a needle.
inocultion needle is used for retrieving solid or dense media and the inoculation loop is used to retrieve liquid media.
they are used to injection of the microscopic organism
unclear question .
To avoid cross-contamination, and indeed all other forms of contamination.
to use needle and cotton means to knit
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
inoculation
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
To inoculate a solid media, a sterilized inoculation loop or needle is used to pick up a small amount of the desired culture. This culture is then streaked onto the surface of the solid media in a specific pattern to ensure isolated colonies grow. The inoculated media is then incubated at the appropriate temperature to allow the colonies to grow.
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.
because you want to introduces as little air as possible
Insert the knitting needle into the loop as if to knit (as opposed to the direction you insert to purl), slip the loop onto the inserted needle without creating a new stitch.
it is like a vibrating needle :3
Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
ewan ko
because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.