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Which antibiotic resistance marker is not used for cloning in gram negative bacteria?
Wiki User
∙ 11y ago
Vancomycin acts by inhibiting proper cell wall synthesis in Gram-positive bacteria. Due to the different mechanism by which Gram-negative bacteria produce their cell walls and the various factors related to entering the outer membrane of Gram-negative organisms, vancomycin is not active against Gram-negative bacteria (except some non-gonococcal species of Neisseria).
The large hydrophilic molecule is able to form hydrogen bond interactions with the terminal D-alanyl-D-alanine moieties of the NAM/NAG-peptides. Under normal circumstances, this is a five-point interaction. This binding of vancomycin to the D-Ala-D-Ala prevents cell wall synthesis in two ways. It prevents the synthesis of the long polymers of N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) that form the backbone strands of the bacterial cell wall, and it prevents the backbone polymers that do manage to form from cross-linking with each other
Wiki User
∙ 11y ago
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Why does the rapid reproduction of bacteria make them a good choice for cloning a foreign gene?
Because bacteria is speshal..:)
What is colning in biology?
Cloning is the process of producing genetically identical offsprings. Naturally, it happens when certain bacteria reproduce asexually..in biotech, it involves creating copies of DNA fragments(molecular cloning) or cells(cell cloning).
Did Ian Wilmut call cloning cloning?
Ian Wilmut did not call cloning cloning. He was the professor that had cloned Dolly (Andrew) the sheep. Johnny
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
What is orchid cloning?
Cloning is a type of asexual reproduction.
Antibiotic in gene cloning?
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
Does a cloning vector contain promoter region?
No, not really since it is just for cloning. But their should be enough promoter/sequence to provide antibiotic resistance.
Describe the use of plasmids as vectors in biotechnology?
Plasmids are often used as expression vectors in biotechnology. Plasmids are small, circular or linear pieces of DNA containing non-essential genes that are found in all life, although much more common in prokaryotes, especially bacteria. These genes confer abilities such as metabolizing a previously unusable compound, building an amino acid previously unbuildable, or even antibiotic resistance. Plasmids are used in research to induce the expression of a gene usually not found in the given organism. For example, you can construct a plasmid with a bacterial promoter connected to the gene for a human protein through a process called 'cloning'. The plasmid with the human gene can then be introduced into bacteria by transforming a competent gram-negative with the plasmid. Usually the plasmid also has an antibiotic resistance gene in addition to the target gene. This antibiotic resistance can be used to select for bacteria containing the plasmid. For example, the most common resistance gene is ampicillin resistance gene. If you grow the transformed bacteria in a culture containing ampicillin, only bacteria containing the antibiotic resistance, and therefore containing the plasmid, can survive. This will ensure that what you have is a pure culture of bacteria containing the plasmid. After selection, these bacteria can be cultured in suitable media to increase their numbers to a point that their production of the human protein becomes appreciable. Then these bacteria are usually lysed (killed) to extract the protein. Sometimes, however, these bacteria can also be made to secrete the protein into the medium.
What are the negative aspects of organ cloning?
Negative aspewccts of organ cloning is that your body might not accept it
Gene cloning can involve inserting genes into?
bacteria
Why does the rapid reproduction of bacteria make them a good choice for cloning a foreign gene?
Because bacteria is speshal..:)
What are the negative side of cloning?
Generally, Cloning has no negative side. Clones can be programmed to obey their duties. But while the clones were being generated, they were made to respond to Order 66, which means- "destroy the Jedi"
What uses bacteria to copy DNA?
They can reproduce very quickly
What is colning in biology?
Cloning is the process of producing genetically identical offsprings. Naturally, it happens when certain bacteria reproduce asexually..in biotech, it involves creating copies of DNA fragments(molecular cloning) or cells(cell cloning).
Negative things of cloning?
becasue pp backs up in your pp hole
What is the small ring of DNA in bacteria?
The small ring of DNA in bacteria is called a plasmid. Plasmids are separate from the bacterial chromosome and can replicate independently. They often carry genes that are advantageous to the bacteria, such as antibiotic resistance genes or genes that allow them to metabolize certain nutrients. Plasmids can be transferred between bacteria through processes like conjugation, allowing for the spread of these advantageous genes.
Why does the capability of bacteria to replicate itself rapidly a useful tool for gene cloning?
Make lots of copies of the gene rapidly.
