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Which fragment moves the furthest in gel electrophoresis?
The smallest fragment will more furthest in the gel.
Would you agree that if a person believes that their smallest DNA fragment is the band nearest the negative pole if the gel?
It is not possible for DNA fragment to be found towards the negative pole of gel. Reason being that the DNA itself is a negatively charged molecule and will always move towards the positive pole when the gel is run. Regarding the smallest fragment, it is impossible to find a band near the negative pole. When the gel is running the smallest fragment runs ahead of all the fragments. It could be found near the positive end, and also possible that if it is too small and the gel is not turned off on correct time then the fragment may overrun the gel from positive end.
Will your D1S80 fragments be separated by gel electrophoresis if you have a 200 base pair and a 400 base pair?
Yes, gel electrophoresis separates fragments based on their size. Therefore it will be able to separate a 200bp fragment from a 400bp fragment provided you use the correct gel composition (as this affects the sensitivity to size differences).
Analysis of DNA fragments in gel electrophoresis involves what?
i)- the size of the DNA fragment, ii)- the density of the agarose gel, iii) - the intensity of the migratory electric field.
What is created by treating DNA with restriction enzymes to create fragments allowing them to travel in an agarose gel bed with electricity and capturing the results in a type of picture?
When DNA is treated with restriction enzymes, and the fragments are loaded onto a gel which is subjected to electrophoresis, we get a banding pattern of the DNA fragments with the farthest band (from the gel) of those fragments smallest in size.
Which fragment moves the furthest in gel electrophoresis?
The smallest fragment will more furthest in the gel.
Why is the largest DNA fragment band found closest to the well in with it was placed?
Because of the molecular weight of DNA, the larger fragment DNA is heavier and cannot move fast through the gel and found near to well whereas the small fragments with small molecule weight can move fast through the gel pores
What is the relationship between the size of the DNA fragment and the distance that it migrates from the well?
. Because DNA is a negatively charged molecule, it will migrate through the gel toward the positive electrode (recall that opposite charges attract). The rate of migration of DNA through the agarose depends on the size of the DNA fragment. The smaller the fragment, the faster it can move through the gel. Another important factor is the concentration of agarose in the gel. The higher the concentration of agarose, the more it slows down the movement of all the DNA fragments.
Would you agree that if a person believes that their smallest DNA fragment is the band nearest the negative pole if the gel?
It is not possible for DNA fragment to be found towards the negative pole of gel. Reason being that the DNA itself is a negatively charged molecule and will always move towards the positive pole when the gel is run. Regarding the smallest fragment, it is impossible to find a band near the negative pole. When the gel is running the smallest fragment runs ahead of all the fragments. It could be found near the positive end, and also possible that if it is too small and the gel is not turned off on correct time then the fragment may overrun the gel from positive end.
What is true of the DNA fragment band closest to the positive end of the gel?
they are the smallest.
What is gel electrophoresis and how is it used to analyze?
Gel electrophoresis is a common method to study DNA. It is a very basic way of comparing the mass (mostly size or length of DNA). The main principle behind gel electrophoresis is that DNA has a slight negative charge. When put in a gel (usually agarose gel) DNA will travel through the gel towards a positive charge, which is generated by the electrophoresis machine. The basic idea behind it, is that DNA will travel through the gel towards the positive pole and away the negative pole of the electrophoresis machine. The smaller the fragment, the further it will travel towards the positive pole, as it will go through the gel quickly. The larger fragments will travel slower towards the positive pole, and will travel less compared to the small fragments. This is how one can quickly compare size of DNA fragments, and possibly even compare between 2 or more DNA strands and find similarities.
Will your D1S80 fragments be separated by gel electrophoresis if you have a 200 base pair and a 400 base pair?
Yes, gel electrophoresis separates fragments based on their size. Therefore it will be able to separate a 200bp fragment from a 400bp fragment provided you use the correct gel composition (as this affects the sensitivity to size differences).
If you have three molecules small medium and large which will travel through gel electrophoresis faster?
Small
What would happen to the DNA fragments if you forgot to turn the current off?
the DNA fragment would keep on runing through gel until they ran off the end.d3aa alamarat
Function of agarose in agarose gel electrophoresis?
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
Analysis of DNA fragments in gel electrophoresis involves what?
i)- the size of the DNA fragment, ii)- the density of the agarose gel, iii) - the intensity of the migratory electric field.
What is created by treating DNA with restriction enzymes to create fragments allowing them to travel in an agarose gel bed with electricity and capturing the results in a type of picture?
When DNA is treated with restriction enzymes, and the fragments are loaded onto a gel which is subjected to electrophoresis, we get a banding pattern of the DNA fragments with the farthest band (from the gel) of those fragments smallest in size.
