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It is not possible for DNA fragment to be found towards the negative pole of gel. Reason being that the DNA itself is a negatively charged molecule and will always move towards the positive pole when the gel is run. Regarding the smallest fragment, it is impossible to find a band near the negative pole. When the gel is running the smallest fragment runs ahead of all the fragments. It could be found near the positive end, and also possible that if it is too small and the gel is not turned off on correct time then the fragment may overrun the gel from positive end.

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Q: Would you agree that if a person believes that their smallest DNA fragment is the band nearest the negative pole if the gel?
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