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What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
What color are gram negative cells?
Gram positive bacterial stain purple. They do not take up the counter stain.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
spore is a dormant structure and necessary to stain because in this we use malachite green which will retain in spore
What is positive staining?
cells which take up crystal violet stain and retain them because of smal pore size
What valuable information can a simple stain of an organism provide?
It can provide every bit of information possible. Ancestory, relatives, organism structure to what they eat.
What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
Why do gram positive bacteria retain the primary stain methylene blue while gram negative bacteria are decolorized by rinsing with ethanol?
capsule
What organism doesn't stain on a gram stain?
Rickettsia
What color is moraxella catarrhalis after the decolorizer is used?
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
What is acid gram?
bacterium that doesn't retain violent stain
What is the purpose of staining an unknown organism?
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.
Which stain gives the best clarity for each organism?
Different types of organisms require different types of staining techniques. The Gram stain is majorly used in the identification of a bacterial organism. This is considered an effective stain for microscopic organisms.
What website could you find facts about stain removers?
which group of cleaners is the best tea stain remover? which group of cleaners is the best tea stain remover?
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
When treated with Gram stain Gram-positive bacteria appear?
Gram positive bacteria retain the violet stain, and appear purplish under a microscope.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
