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The cell wall.. gram stain tests for presence of a thick outer layer of peptidoglycan.

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13y ago
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8y ago

It is the cell wall. Cell wall get stained.

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The thick cell walls of Gram-positive bacteria retain such stains as the crystal violet-iodine dye in the cytoplasm. Gram- will not.

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Cell wall is stained by it. Cell wall is colorized

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Q: Which part of bacteria cell is most involved in gram stain?
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Does the gram stain procedure work for all bacteria?

Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.


Can you gram stain a cell without a cell wall?

No bacteria exist without a cell wall! If they loose cell wall then they die. Gram staining is used to differentiate gram positive (that doesnt have outer membrane) and gram negative (cell wall+outer membrane) bacteria.


What is the color of Gram positive bacteria and why?

Gram positive bacteria stain purple due to the chemistry and structure of their petidoglycan cell wall


Which structure of bacteria is determied by gram staining?

The part of the bacterial cell that appears to play the most important role in determining whether an organism if gram-positive or not is the cell wall. it is determined by whether or not it retains the stain during testing.


What does a gram reaction of a cell refer to?

the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.

Related questions

Does the gram stain procedure work for all bacteria?

Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.


What is the Gram stain for measles?

Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.


Why would you want to know if a gram stain is positive or negative?

Gram negative bacteria (pink gram stain) contain no outer cell membrane, while gram positive bacteria (purple gram stain) do contain an outer cell membrane. Gram negative and positive bacteria can respond differently to antibiotics. Many only work on only one of the two bacteria types. A gram stain is also the first step in identifying a bacteria, dividing bacteria into two large and distinct groups.


What is the gram stain for measles virus?

Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.


Can you gram stain a cell without a cell wall?

No bacteria exist without a cell wall! If they loose cell wall then they die. Gram staining is used to differentiate gram positive (that doesnt have outer membrane) and gram negative (cell wall+outer membrane) bacteria.


What is the difference between gram-positive and gram-negative bacteria?

The presence of thick cell wall. Gram positives have a thick cell wall and remain blue. Gram negatives have a thin cell wall where the blue stain washes out and then will stain pink with the counter stain.


Can you tell an organisms gram stain by simple staining?

Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).


Why does some bacteria stain purple and some red?

The purple stained bacteria are called gram positive bacteria and retain the stain after washing by having a thick cell wall one one layer of a heavily crossed linked material called peptidoglycan. ( Google that ) The red stained bacteria are gram negative because they posses two thinner layers of peptidogylcan cell wall that that do not hold the gram stain and it is mostly washed away in rinsing the bacterial samples.


Which thing make gram positive bacteria to get stain?

the smooth part of the cell wall make it to get stain


What are the uses of the gram stain technique scientifically speaking?

Gram stains are used to analyze bacteria and determine their characteristics. The most popular use of a gram stain is to determine the thickness of a cell wall in bacteria.


What does gram negative on a serratia marcescens gram stain mean?

Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.


What is the difference between gram plus and?

Gram positive bacteria stain purple with the Gram stain. This is because of the fact that they contain a thick layer of murein in their cell wall, which takes up the stain very well. Gram negative bacteria, however, do not display the thick layer of peptidoglycan on their outer surface. Therefore, they stain red with the counter stain.