It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. Bacterial cells, obtained from the culture (liquid culture or colonies grown on agar plate), is resuspended in this buffer. The purpose of this buffer is to provide an optimal starting pH (pH 8.0) and an ideal condition for subsequent lysis.
It cooprates with the lysis buffer to make our DNA sample visible to the human eye! lol jk.
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
reduce secondary structure
It is used as a cryo-protectant. DMSO prevents the formation of ice crystals and prevents cell lysis during thawing.
MgCl2 is added to the lysis buffer since Mg2+ ions are co-factors for the enzyme used in the lysis buffer. This enzyme requires magnesium ions in order to function properly.
In lysis buffer urea denature the protein and increase the solubility of protein.
A lysis buffer is a solution which is used to breakdown or separate the components of cells. Like all buffers, it is supposed to maintain the pH within a narrow range. Lysis buffers are used when analysis of separate components of the cell as desired - such as DNA isolation.
to inhibit divalent cation-dependent proteases
We use it for isolation of proteins from yeast cells as a lysis buffer
The composition of Buffer P2 is:200 mM NaOH1% SDS (w/v)Buffer P2 is the lysis buffer
Triton X-100 is used as a lysis buffer for DNA separation.
In lysis buffer, the function of ammonium chloride is to determine the red blood cells in samples. These samples also contain white blood cells.
Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. Bacterial cells, obtained from the culture (liquid culture or colonies grown on agar plate), is resuspended in this buffer. The purpose of this buffer is to provide an optimal starting pH (pH 8.0) and an ideal condition for subsequent lysis.
for cell lysis
To protect protein during thawing and freezing