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Chemical equaton of reaction between glycine and ninhydrin solution?
nee amma puku
What color will be produced by proline with ninhydrin?
When ammonia or primary and secondary amines are detected, a deep blue or purple color known as Ruhemann's purple is produced. Ninhydrin can also be used to monitor deprotection in solid phase peptide synthesis (Kaiser Test). The chain is linked via its C-terminus to the solid support, with the N-terminus extending off it. When that nitrogen is deprotected, a ninhydrin test yields blue. Amino-acid residues are attached with their N-terminus protected, so if the next residue has been successfully coupled onto the chain, the test gives a colorless or yellow result.
What functional group must be present for the ninhydrin test to work?
Ninhydrin works because it reacts with the amino acids left behind in a latent print. Ninhydrin has a polar carbonyl carbon with is electron deficient. It is attacked by the nucleophilic nitrogren on an amino acid, temporarily combining the ninhydrin and amino acid molecule. The structure is rearranged until the origionally attacked carbon is protonated and leaves in the form of water. This creates a schiff base when the nitrogen is double bonded to the origionally attacked carbon. This molecule rearranges again so that the nitrogen is double bonded to the adjacent carbon of the amino acid. This last rearrangement produces carbon dioxide gas. Further rearrangement of the product produces ruheman's purple.
Why you will spray ninhydrin for analysis in chromatography?
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
Result of casein in ninhydrin test?
Kharkov's
Is Ninhydrin used to test for the presence of lipids?
No, a grease spot test or Sudan IV test can be used to test for lipids. Ninhydrin is used to test for amino acids.
Why will ninhydrin cause blue-purple stains on skin?
I'd have described them as more of a yellowish-orange, myself. Ninhydrin reacts with amino acids. Amino acids are all over your skin. With most of them it forms a yellowish compound; with a couple it's more of the blue-purple you're describing.
Is ninhydrin the same as super glue?
NO, ninhydrin is not the same as super glue
Chemical equaton of reaction between glycine and ninhydrin solution?
nee amma puku
What color will be produced by proline with ninhydrin?
When ammonia or primary and secondary amines are detected, a deep blue or purple color known as Ruhemann's purple is produced. Ninhydrin can also be used to monitor deprotection in solid phase peptide synthesis (Kaiser Test). The chain is linked via its C-terminus to the solid support, with the N-terminus extending off it. When that nitrogen is deprotected, a ninhydrin test yields blue. Amino-acid residues are attached with their N-terminus protected, so if the next residue has been successfully coupled onto the chain, the test gives a colorless or yellow result.
Does ninhydrin has flaws?
Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.
What substances are found on the human skin that react with ninhydrin resulting to stain or violet coloration on the skin?
Ninhydrin (2,2-dihydroxyindane-1,3-dione) is a substance intended to discover the existence of Ammonia or anything that contains Amines. When this substance reacts with these Amines a blue-purple stain will be observed. Since, the skin contains amino acid, a blue-purple stain will be noticed.
What functional group must be present for the ninhydrin test to work?
Ninhydrin works because it reacts with the amino acids left behind in a latent print. Ninhydrin has a polar carbonyl carbon with is electron deficient. It is attacked by the nucleophilic nitrogren on an amino acid, temporarily combining the ninhydrin and amino acid molecule. The structure is rearranged until the origionally attacked carbon is protonated and leaves in the form of water. This creates a schiff base when the nitrogen is double bonded to the origionally attacked carbon. This molecule rearranges again so that the nitrogen is double bonded to the adjacent carbon of the amino acid. This last rearrangement produces carbon dioxide gas. Further rearrangement of the product produces ruheman's purple.
What is the molecular formula of ninhydrin?
Well, the best I could come up with is it's either:C9H6O2orC4H3O2If someone knows any different please correct me.jman63: it is actually C9H6O4
What group in amino acids or proteins in general is responsible for the ninhydrin reaction?
It would be the amine group. In fact, ninhydrin will react with other amines, not necessarily those in proteins or amino acids. it reacts with free amines to produce blue-purple color and yellow in proline.
Why you will spray ninhydrin for analysis in chromatography?
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
How can you spot a link?
it is blue
