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Antibiotics trigger a protective response by bacteria to form biofilms. Bacteria within a biofilm are thousands of times less susceptible to antibiotics than planktonic bacteria
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Is biofilm sensitive to antibiotics?
Biofilms are generally thousands of times less sensitive to antibiotics than planktonic bacteria.
Which are the stages of the biofilm formation?
Formation of a conditioning film Non-permanent attachment Permanent attachment Growth & maturation Dispersal
What two factors make a biofilm in an intravenous line especially dangerous to the patient?
The two factors that make biofilm in an intravenous line especially dangerous to the patient is infection and the difficulty of treatment. While the microorganisms are within the biofilm, they cannot be eradicated with antibiotics. However, if the biofilm were to detach from the IV line, the access to the circulatory system would potentially cause a massive infection.
Is a natural biofilm a pure culture?
A biofilm may consist of a single species embedded in extracellular polymeric substance (EPS), or it may consist of multiple species. The monospecies biofilm does constitute a 'pure' culture, and these do occur under natural circumstances, as for example, H. pylori biofilm in the human stomach (Cole et al (2004) Characterization of Monospecies biofilm formation by Helicobacter pylori," Journal of Bacteriology 186:3124-3132). However, most biofilms that people are familiar with - pipe slime, tooth plaque, etc. - include multiple bacterial species, and can include algae and/or fungus.
How does biofilm work?
A biofilm is a group of microorganisms in which cells stick to each other. The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. If the colonists are not immediately separated from the surface, they can anchor themselves more permanently using cell adhesion.
What are the benefits of biofilm made by normal flora?
Longevity.
Where doesnt a biofilm occurs?
Sterile or dry conditions
What is biofilm?
Biofilm forms when bacteria sticks to surfaces in moist environments by excreting a slimy substance. Examples of this would be the slippery rocks on the bottom of a stream or plaque on teeth.
Which microscope would be MOST useful for examining the contours of the surface of a bacteria cell?
It depends on the type of biofilm, what surface the biofilm is on, and what information you want to get by looking at the biofilm under a microscope. If you just want to look at how much of a surface is covered by a biofilm, you can use normal light trasmission microscopy (as long as the surface is transparent e.g. glass). Alternatively you could use epifluorescent microscopy in combination with a fluorescent stain. If you want to look at the structure of the biofilm, confocal laser scanning microscopy is probably the best as you can get a 3D image. Other useful types of microscopy include phase contrast and DIC, which allow you to look at the biofilm without staining it first.
What is the term used for a biofilm that builds up on the teeth?
plaque
What are actions of antibiotics?
(1) Neutralization (2) Opsonization (3) Precipitation (4) Adherence inhibition (5) Immune complex formation
Which microscope is most useful for visualizing a biofilm?
scanning acoustic microscope
