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Mouse embryonic fibroblasts are inactivated by either chemical (Mitomycin-C) or radioactive (Cs-137) means before culturing with embyronic stem cells to prevent the fibroblasts from merging with the stem cells and creating a chimeric cell.

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Q: Why are mouse ES cells Cultured on Irradiated Mouse Embryonic Fibroblasts?
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Related questions

What is PMEF?

Primary Mouse Embryo Fibroblasts


Where in a mouse would you find fibroblast and how could I get it?

Lol "fibroblasts" they are cells


What has the author Mehran Sam written?

Mehran Sam has written: 'Gene trap studies of the mouse genome and embryonic development' 'Advance directives'


What has the author Ivonne Lastra written?

Ivonne Lastra has written: 'A study of the development of mouse embryonic tissue transplanted into the chorio-allantoic membrane of the chicken embryo, with special reference to the development of the embryonic heart' -- subject(s): Heart, Mice, Embryology, Chickens, Embryos


What has the author Ella Korets-Smith written?

Ella Korets-Smith has written: 'Embryonic stem cell-based technology to study gene function in the mouse'


New calls are priduced within bone marrow as a direct result of?

If bone marrow cells from the transplanted mouse can, in turn, be transplanted able to direct human embryonic stem cells.


What is NIH T3T cell line?

I belive you mean the NIH 3T3 cell line which is the mouse embryonic fibroblast cell line, capable of differentiating into multiple cell types - adipocytes, myocytes or chondrocytes.


Is a chimera mouse model an actual living animal?

Yes it is, but it is one that was created by science. The chimera mouse is not an evolved species. It is cute, all right, just like any other mouse but, unlike most other mice, it was created by scientists who injected embryonic stem cells from a wood mouse into an early embryo belonging to a house mouse. Doesn't sound that amazing? Please note that the species house mouse and wood mouse have less genetic material in common than those of human and chimpanzee. There are a few of these little guys around, but do not expect them to be wandering your walls. They live under laboratory conditions and are explained in more detail at: http://www.sciencedaily.com/releases/2007/10/071019093241.htm


How do you know if a baby mouse is a mouse?

Is the mother a mouse? Is the father a mouse? Then yes, it is a mouse.


What shares a common ancestor with a chimp and a mouse?

All modern mammals. And most probably all animals who share the basic four-limbs-one-head-two-eyes-one-mouth body schematic during embryonic stage (fish, reptiles, and birds) according to studies of embryos.


What kind of mouse?

Danger mouse, jerry mouse, micky mouse... take your pick!


Who is Mickey Mouse's girlfriend?

Minnie Mouse is Mickey Mouse's girlfriend.