0
Why did we cut both segments of DNA with the same restriction enzyme?
Wiki User
∙ 9y agoWant this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
Why is it essential that the same restriction enzyme be used to create a transgenic organism?
It is essential so that the enzyme does not cut out nucleotides that is not supposed to.
Does a restriction enzyme generate the same size fragments in genomic DNA of different species?
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
What is the function of restriction enzymes in the process of DNA recombination?
First, a specific enzyme is needed to cut the DNA from the donor genes at a specific site. This enzyme is called a restriction enzyme.The enzyme is used to cut out a piece of DNA that contains one or more desired genes from the donor's DNA. Next, a vector is needed to receive the donor DNA. Most frequently, a naturally occurring circular piece of bacterial DNA, called a plasmid, is used for this purpose. Finally, an enzyme is used to "stitch" the donor DNA into the plasmid vector. This enzyme is called ligase, and it creates permanent bonds between the donor DNA and the plasmid DNA. The result is that the donor DNA is incorporated into the bacterial plasmid, forming the recombinant DNA (rDNA)
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
Why is it essential that the same restriction enzyme be used to create a transgenic organism?
It is essential so that the enzyme does not cut out nucleotides that is not supposed to.
Does a restriction enzyme generate the same size fragments in genomic DNA of different species?
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
Can parallel lines be line segments?
They can not be line segments on the same line, but they can both be line segments.
What is the function of restriction enzymes in the process of DNA recombination?
First, a specific enzyme is needed to cut the DNA from the donor genes at a specific site. This enzyme is called a restriction enzyme.The enzyme is used to cut out a piece of DNA that contains one or more desired genes from the donor's DNA. Next, a vector is needed to receive the donor DNA. Most frequently, a naturally occurring circular piece of bacterial DNA, called a plasmid, is used for this purpose. Finally, an enzyme is used to "stitch" the donor DNA into the plasmid vector. This enzyme is called ligase, and it creates permanent bonds between the donor DNA and the plasmid DNA. The result is that the donor DNA is incorporated into the bacterial plasmid, forming the recombinant DNA (rDNA)
Why do you use the same restriction enzyme when you splice together two separate things?
Using the same restriction enzyme when splicing DNA into plasmids, etc., is effective as restriction enzymes are site-specific. Therefore, the spliced DNA will be able to complementary base pair with the ends of the spliced plasmid due to the identical recognition sites. Since the two molecules have the same sticky ends, they will be able to fit together.
What types of DNA sequences do restriction enzymes recognize?
Every restriction enzyme has a very specific sequence that it will recognise. Quite often, but not always, these sequences are palindromic (meaning they repeat backwards, like words that read the same backwards and forwards). The exact nature of the DNA sequence depends on the restriction enzyme (there are many different ones).
How dimerization takes place?
when the two strands or adopters are cutted with same restriction enzyme and they are complementary to each other, they attached and recircularized.
How is a cube and a cylinder the same?
they both have faces and line segments
What does it mean for angles and segments to be congruent?
Two angles are congruent if they both measure exactly the same number of degrees. Two line segments are congruent if they both have exactly the same length.
Segments that have the same length?
The answer to, "Segments that have the same length" Is Congruent Segments!
