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Why is it essential that the same restriction enzyme be used to create a transgenic organism?
Wiki User
∙ 14y ago
It is essential so that the enzyme does not cut out nucleotides that is not supposed to.
Wiki User
∙ 14y ago
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How do restriction enzymes help make recombinant DNA and transgenic organisms?
Restriction enzymes take apart the DNA in a certain area and allow for a plasmid to be inserted within the gap that is created. Restriction enzyme use is basically the same for both the production of recombinant DNA and for transgenic organisms since an organism can synthesize the DNA that has been inserted into it once it has been placed within the organism. In the case of unicellular organisms the restriction enzyme is first introduced to break apart the DNA and then the plasmid is introduced to create the desired effect. Then the organism can express those genes through further processing of the newly introduced DNA and through mitosis (which is how unicellular organisms reproduce) it can give that gene to its offspring. In the case of multicellular organisms a restriction enzyme and accompanying plasmid must be presented when the organism is just a zygote. This process is how those glow-in-the-dark fish are created and provided that those fish can reproduce they'll also give their traits on to future generations just like single-celled organisms would.
How are transgenic mice created?
a transgenic species is created by an gentic material. This is known as DNA molecules, which are combined into one molecule to create a new set of genes
What is a restriction map?
A restriction map plots restriction sites within a chain of DNA. You cannot create a restriction map without restriction enzymes. Restriction sites are points in a DNA molecule that contain certain strings of nucleotides, which can only be identified by restriction enzymes.
What is the importance of transgenic organisms?
A genetically modified organism (GMO) or genetically engineered organism (GEO) is an organism whose genetic material has been altered using genetic engineering techniques. These techniques, generally known as recombinant DNA technology, use DNA molecules from different sources, which are combined into one molecule to create a new set of genes. This DNA is then transferred into an organism, giving it modified or novel genes. Transgenic organisms, a subset of GMOs, are organisms which have inserted DNA that originated in a different species
What organism is needed to create photosynthesis?
A plant
How do restriction enzymes help make recombinant DNA and transgenic organisms?
Restriction enzymes take apart the DNA in a certain area and allow for a plasmid to be inserted within the gap that is created. Restriction enzyme use is basically the same for both the production of recombinant DNA and for transgenic organisms since an organism can synthesize the DNA that has been inserted into it once it has been placed within the organism. In the case of unicellular organisms the restriction enzyme is first introduced to break apart the DNA and then the plasmid is introduced to create the desired effect. Then the organism can express those genes through further processing of the newly introduced DNA and through mitosis (which is how unicellular organisms reproduce) it can give that gene to its offspring. In the case of multicellular organisms a restriction enzyme and accompanying plasmid must be presented when the organism is just a zygote. This process is how those glow-in-the-dark fish are created and provided that those fish can reproduce they'll also give their traits on to future generations just like single-celled organisms would.
Who creates a deed restriction?
The owner of the land can create a deed restriction.The owner of the land can create a deed restriction.The owner of the land can create a deed restriction.The owner of the land can create a deed restriction.
How are transgenic mice created?
a transgenic species is created by an gentic material. This is known as DNA molecules, which are combined into one molecule to create a new set of genes
What is the process of making a genetically modified organism in a lab?
Contrary to the common understanding of the word, the process which we use to create transgenic organisms is called "cloning". This doesn't refer to copying an organism as often believed. Cloning as scientists mean it refers to creating DNA which does not naturally occur in any organisms. This is also called transgenic DNA.To make a genetically modified organism, we need:transgenic DNA - made by cloningan organismThe transgenic DNA of course needs to be made for a specific purpose. Depending on how big it needs to be, it can be made in different ways. Very small stretches of DNA can be synthesised chemically. Larger bits of DNA are usually made by extracting a cell's DNA and then using a technique called polymerase chain reaction (PCR) to take only one particular bit of the DNA out. We then use restriction enzymes and ligase to cut and paste different bits of DNA together. Usually, the end product will be a plasmid.The finished plasmid (transgenic DNA) then needs to be introduced into the target organism - and there are different ways of doing it for every type of organisms. Some bacteria for example will simply take up plasmids and integrate them into their own DNA without a complicated process around it being necessary. For bigger organisms like animals, it's very difficult - usually impossible - to modify an already living organism. For these, the only option is usually to take an oocyte (egg cell, e.g. a flower seed) and introduce the plasmid into it. If the egg survives this procedure and develops (which is very difficult to achieve), the resulting organism will be transgenic, or genetically modified.
How are transgenic animals used in the livestock industry?
Currently, they are not. A transgenic animal is one that has been genetically modified to carry a gene that is not normally found in that species. There are a few livestock animals that are transgenic, but they are carefully monitored in research facilities because they are quite expensive to create and to maintain.
What is a restriction map?
A restriction map plots restriction sites within a chain of DNA. You cannot create a restriction map without restriction enzymes. Restriction sites are points in a DNA molecule that contain certain strings of nucleotides, which can only be identified by restriction enzymes.
Why are some human proteins produced by transgenic cows?
Some transgenic cows are engineered to produce human proteins to create substitutes or other alternatives to such things like breast feeding, baby formula and non-transgenic cow milk. They also are created to be an alternative for people who have specific protein allergies.
What is the importance of transgenic organisms?
A genetically modified organism (GMO) or genetically engineered organism (GEO) is an organism whose genetic material has been altered using genetic engineering techniques. These techniques, generally known as recombinant DNA technology, use DNA molecules from different sources, which are combined into one molecule to create a new set of genes. This DNA is then transferred into an organism, giving it modified or novel genes. Transgenic organisms, a subset of GMOs, are organisms which have inserted DNA that originated in a different species
Create a model of a fictitious organism that is adapted to its environment.?
Create a fiction organism and how it lives or relates to its enviornment
Why must web pages be created by organizations?
There is no restriction on who can create web pages
When you take a cell from one organism and create an identical organism it is called what?
cloning
What organism is needed to create photosynthesis?
A plant
