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They're presumably the controls. You need to treat them exactly the same as the others, except for one specific thing. In this case, it's that they were never opened.

Otherwise, you wouldn't be able to know for certain whether the difference in the results was because you didn't open them or because you didn't incubate them.

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Q: Why did you have to incubate the agar plates which were never opened during the ubiquitous microbes exercise?
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