Want this question answered?
Agar is used to culture bacteria for scientific research.
Agar plate method is used to culture bacteria in lab .
Gram negative gastrointestinal bacteria, colonies such as E.coli can usually be isolated on simple agar plates, incubated at roughly 37 degrees. Other plates can be used if you want to isolate certain aspects of the colony, but usually agar is the first choice.
An agar plate was flooded with a culture of a species of bacterium usually found in the mouth. Four steriled paper discs, A, B, C, and D, each containing a different brand of mouthwash, were then placed on the agar plate. The drawing shows the appearance of the plate after it had been incubated below body temperature for three days, this is to ensure that the bacteria are not harmful to humans. Describe the aseptic technique that would be used when flooding the agar plate with bacteria
Because plate count agar is still agar. therefore, it still has all the nutrients that bacteria require to grow.
Agar plates gives you a more visual view of the bacteria growth but is limited in the amount of bacteria that can grow on the plate. With broth, you won't be able to see the bacteria colonies but you will be able to grow much more of the bacteria for sampling.
to inoculate an agar plate you would place bacteria on the agar to grow
There are areas on the agar plate where no bacteria grow because that area was missed when the plate was streaked.
An agar plate should be placed upside down in the incubator to keep water drops off the agar and prevent the bacteria from drowning. The bacteria are incubatored at 30-40*c for one to three days. the bacteria feed from the nutrients in the agar and reproduce to form colonies of millions of bacteria. Source: NCEA level one science study guide 1.3 biology AS 90188
How do colonies on the surface of a pour plate differ from those suspended in the agar?
When bacteria is grown in an Agar plate, one quantitative method to measure growth is using a counting chamber. Another method is using viable plate counts.
Agar is used to culture bacteria for scientific research.
it is used to count the colonies
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
How do colonies on the surface of a pour plate differ from those suspended in the agar?
Agar plate method is used to culture bacteria in lab .
Use selective media agar plates. Different types of agar will let bacteria grow and inhibit fungal growth, or vice versa.