we can use feulgen's method ,in which malachite green stain is used to stain the nucleus
the purpose of using stain is to highlight specific areas or parts of the cell. different cell structures become visible under different stain colors.
There is no complex chemical reason why iodine is added to a cell. It is simply added to stain a cell to make it easier to study under a microscope, as it gives some contrast to the cell to make certain organelles stand out that would be unclear or invisible without this stain. There are, however, many other stains that can be used, for example Haematoxyline and Eosin (H&E) staining which is used often to identify and study cancerous cells.
When you stain a Eukaryotic Cell, the nucleus becomes more clear visually. This makes it more useful to stain a Eukaryotic Cell than a Prokaryotic Cell because Prokaryotic Cells do not have a true Nucleus. Hope it helped! -iKiNG
This part of the nucleus is the nucleoplasm.
The whole cell doesn't stain during a cell wall stain because the dyes that are used are only attracted to the negative cell wall and only sticks it. The inside of the cell shows clear.
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
In a gram positive stain it is because the cell's cell wall is made up of peptioglycan
An azurophile is a cell which has been readily stained with an azure stain.
Saffranin is a " counter stain " . this is also a " basic dye " which is in different color than primary stain ( basic dye crystal violet ) . The purpose counter stain ( saffranin ) is to give the decolourised cells a color , that is different from first one ( crystal violet ). The microbes which are not decolourised by alcohol retains the stain, while the decolourised cell take up the counter stain ( saffranin ).
Eosinophils
You can observe cheek cells
The presence of thick cell wall. Gram positives have a thick cell wall and remain blue. Gram negatives have a thin cell wall where the blue stain washes out and then will stain pink with the counter stain.
we can use feulgen's method ,in which malachite green stain is used to stain the nucleus
Safranin is a biological stain which generally colors the cell nuclei red. In analytical chemistry, it is also used as redox indicator.
A simple stain like iodine can make cell parts show up that would otherwise be nearly invisible since they are colorless.A simple stain like iodine will reveal a cell's morphology.
the purpose of using stain is to highlight specific areas or parts of the cell. different cell structures become visible under different stain colors.