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What is the correct order of experimental steps when extracting hemoglobin from red blood cells?
Separate cells from plasma, lyse cells, precipitate protein, dialyze protein sample
Which method is better for separating mixture evaporation or filtration?
If you have a solid precipitate /liquid mixture filtration is the better method of recovering the solid as any contaminant in the liquid can potentially be washed out. Evaporating the liquid to remove it would just deposit whatever residues there were onto the precipitate.
What Physical properties could be used in the method of continuous variation?
temperature, color change, precipitate formed
When does proteins precipitate?
Protein molecules are stabilized its structure by various non covalent interactions. When proteins exposed to advers pH or temperature (high or low) that are not favorable to its stability, they precipitate out from the buffer. The precipitated proteins generally lose its biological activity.
Using benedict's reagent what method is used to find protein?
If the color is blue, it means that protein is present, if it turns orange, protein is not present.
Is lowry's protein estimation an invasive method?
No. It is a non-invasive method
Which method is best for determining Protein Quality?
Lowry method
Why protein estimation is done at 660 nanometer in folin lowry method?
because folin lowry reagent has absorption maxima at 660 nm
How long the blue colour is stable in protein estimation by lowry's method?
60 minutes 60 minutes
Advantages and disadvantages of Lowry's method?
Lowry's method tests for the total amount of protein in a solution. An advantage is that it is quick and easy to do with the color changes being readily recognizable. A disadvantage is that cysteine residue can create errors in the test.
If I am given an unknown protein shake in an unlabeled bottle what could i do to determine the percentage of protein in the shake?
Add vinegar and measure the protein precipitate. Mass of precipitate/mass of shake x100= % protein
Is lowry's method an invasive method?
No. It is a non-invasive method
Why 75 percent ethanol is used in rna extraction?
to precipitate protein.
What has the author Rachel Helen Lowry written?
Rachel Helen Lowry has written: 'A physiological method of the human cough reflex'
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
What is the method of Bence Jones Protein in the urine test?
put 5ml of concentrated hydrochloric acid in a clear tube.carefully add 3ml of test urine to make a good interface without mixing the two.a white curdy precipitate at the interface indicates presence of bence Jones protein
What is the order of steps when extracting hemoglobin from red blood cells?
Separate cells from plasma, lyse cells, precipitate protein, dialyze protein sample.
