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Q: Why does a inoculate citrate slant and incubate for 48 hours change from green to blue?
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Why the slant portion is blue but the butt remains green at the microorganism grows on Simmon's citrate agar?

Fermenting citrate


What is the result of citrate test for bacillus licheniformis?

The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.


Does pseudomonas aeruginosa have a positive result for citrate utilization?

The color changes from the slant's original green to blue, indicationg that the species is able to utilize citrate as a carbon source. Thus, it is POSITIVE


What is the CLSI and ASPT correct order of draw?

yellow,blue black red green purple grey dark blue


What is simmons citrate agar?

Simmons citrate agar is a differential agar used to determine if a sample bacteria can utilize citrate as its only carbon source. The agar is initially a green color due to the bromo thymol blue pH indicator in it. If a bacteria uses the citrate, the by-products are ammonia and ammonium hydroxide both of which will alkalize the agar and increase the pH to the point of changing the indicator's color to blue, so the whole agar turns from green to blue.


Citrate utilization test?

The test indicates if an organism utilizes Citrate as it's only source of Carbon. Positive results will show growth on slant as well as changing the medium from green to blue (ph indicator)


Why is the citrate test carried out on an agar slope rather than a broth?

Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.


What is the CLSI and ASPT correct order of draw when using a butterfly?

yellow,blue black red green purple grey dark blue


Is Pseudomonas luteola oxidase positive?

in the absence of glucose or lactose , some bacteria are able to use citrate as only source of carcon. alkaline pH change the bromothymol blue indicator from green to blue. pseudomonas can not ferment the glucose and the lactose so it use the citrate as carbon source.


Can taking magnesium citrate cause your urine to be green yellow for a day after taking?

i have experienced personally that even few tablets of magnesia phos 6x can cause yellow to yellow green urine which were previously pale.


What vacutainer would you use for a blood chemistry test?

A centrifuge tube would be useful, but a regular test tube will suffice.


Why is ammonium in soft drinks?

Ammonium ferric citrate is a food additive with E number E381 used as an acidity regulator. It is a green or reddish-brown powder which is very soluble in water.