Because it is a type of stain that uses contrasting (in this case the presence or absence of a stain) color to determine what type or classification the organism is. Differential = determining between different groups; you are staining to differentiate one group from another by classifying the organisms' ability to quickly resist decolorization by acids.
First - The purpose of staining (in general) is to enable one to see cells microscopically by adding color to the cell. This is due to the fact that unstained bacteria are transparent and are difficult to observe. Acid fast stain is a differential staining procedure (uses more than one stain). This stain is used to distinquish species of MYCOBACTERIUM (the causitive agent of tuberculosis and Leprosy).
Because in a culture of bacterium, it stains the bacteria that are pathogenic (disease causing).
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Acid fast stains are used to stain microbacters (such as tuberculosis) that possess a thick, waxy cell wall that isn't completely stained by a normal staining process.
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The heat is the mordant for an acid-fast stain
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
So few organisms are acid-fast, the acid fast stain is used only when infection by an acid-fast organisms is suspected.
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
Safranin
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
modified kinyoun's acid fast stain (cold)
The heat is the mordant for an acid-fast stain
The carbolfuchsin is a differential stain. It is an acid-fast stain which distinguishes organisms with waxy cell walls with the use of acid alcohol.
Acid-fast bacteria stain pink. Non acid-fast bacteria stain green.
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Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
So few organisms are acid-fast, the acid fast stain is used only when infection by an acid-fast organisms is suspected.
purple
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
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