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Why should nutrient agar be cooled to 50 c before inoculating the bacteria?
Wiki User
∙ 9y ago
To minimise condensation on the lid of the Petri dish.
Wiki User
∙ 15y ago
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Why is the loop allowed to cool before putting it into the bacterial culture?
Because too much heat can kill bacteria. Think of pasteurised milk - the pasteurising process involves rapid heating and cooling to kill off any bacteria that might be present in the milk.
Why is culture medium cooled 48 temperature before is poured?
In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.
Why boil water and be cooled before getting the pH?
The pH depends on the temperature.
Where online can a person purchase an air cooled chiller?
One can purchase an air cooled chiller online in stores like Amazon or eBay. One can look at customer reviews there before purchasing and select the best fitting model.
Can milk be heated cooled and reheated then cooled?
Yes
Why cooling the inoculating instrument prior to obtaining the inoculum?
The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.
Why should you let the inoculating loop cool first before and after using it?
The inoculating tube is cooled before use so that the organism is not killed by extensive heat.
Why must the inoculating loop be cooled before touching a culture?
To avoid killing the test subjects before the test can begin.
Why must the inoculating loop be cooled first before touching a culture?
To avoid killing the test subjects before the test can begin.
Why is the milk cooled before the bacteria are added?
Because it is. face it
Why is the loop allowed to cool before putting it into the bacterial culture?
Because too much heat can kill bacteria. Think of pasteurised milk - the pasteurising process involves rapid heating and cooling to kill off any bacteria that might be present in the milk.
What will result when 1 to 5 agar is added to nutrient broth boiled and cooled?
Solid medium
What will result when 1 percent to 5 percent agar is added to nutrient broth boiled and cooled?
a solid medium
Should food be cooled down before putting it fridge?
Yes, from what I have heard and read. Moving hot food to a cold refrigerator can cause bacteria to form and grow rapidly. The food should be cooled to room temperature before refrigerating.
Does cold rice have bacteria in it?
It depends how long it was left at room temperature before being refrigerated. It is, surprisingly, a food with a high risk of carrying food poisoning bacteria, as it can hold the bacteria at slightly-higher-than-room-temperature (their optimum breeding temperature) for longer than many other foods. Once cooled, the bacteria are still there.
Why should you let inoculating loop cool first before using?
an innoculated loop shouln't be hot. it should be cooled before contact with the organism by touching it off the edge of the agar or dipped into the top of the broth. if its hot it will kill the organism!
What are the other inoculating instruments aside from needle?
Aside from the inoculating needle, an inoculation loop, is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. Its tip is a wire made of platinum or nichrome, the latter being inferior but less expensive. The wire forms a small loop with a diameter of about 5 mm. This loop is handy for taking an inoculum from a liquid by using the phenomenon of surface tension. It is also called a smear loop, inoculation wand or microstreaker, The loop is used to cultivate microbes in Agar jelly and to use the streak maneuver to transfer microbes. The inoculation loop is always sterilized in a flame until it becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. After flame sterilization, the loop must be cooled so that the next cells to touch the loop don't instantly die.
