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Why is it advice to work near the flame during aseptic technique?
Wiki User
∙ 12y ago
It is advice to work near a flame during aseptic technique because it has to be performed under sterile conditions. Working near a flame allows for flame sterilization which would promote sterilization to the experiment you are working on.
Wiki User
∙ 12y ago
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What is meant by streak dilution technique?
The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this; 1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock. 2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first. 3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock) 4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock) 5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock) 6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible. The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.
What are Two kinds of flame?
the yellow/safety flame - thats the one that burns less the blue flame - burns THE ROARING FLAME - that one burns a lot and you can tell the difference from the blue flame because it makes a roaring sound
What type of a flame is best to use?
if you are talking about what flame to use on a Bunsen burner than the yellow flame is to make sure that everyone knows that there is a flame but the blue flame (less visible) is used as the hotter flame and the better one.
Is flame an abstract noun?
No, the noun flame is usually a concrete noun, a word for a thing that can be seen, touched, sometimes smelled, and even measured for size and temperature; a flame is a physical thing. The noun flame is sometimes used in an abstract context, for example to refer to an 'old flame', or the 'flame of desire'. The word flame is also a verb (flame, flames, flaming, flamed).
How do you increase the temperature of a yellow flame coming out of a busen burner?
You can't it is always the same. You only use the blue flame to heat things because the yellow flame is the safety flame and the blue flame is hotter.
Flaming an inoculating loop?
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
What do you call the technique when the flame comes out when the chef is cooking?
flambe..
What is the purpose of ethanol in the spread plate technique?
There are mainly two techniques in microbiology in bacterial cell inoculation. The first is when the colony is added to the plate and spread with a spreader across the entire plate in aseptic conditions. The second is called 16-streak and is used to isolate a single colony
What is smear preparation?
A smear preparation is a techniques that is used as a precursor is many different staining techniques.The smear prep usually calls for the sample bacterium to be placed on a microscope slide (use aseptic technique) with one drop of DI water. Then the slide is allowed to air dry for a few minutes then the slide is passed through a Bunsen burner flame. Use a cloths pin so that you do not burn you hands Only pass the glass microscope slide through the flame not you cloths pin if it is wood it will catch fire. Pass the slide through 3-4 times then you are ready to continue on with your desired staining technique
What is sterile technique what dose this involve when should this be used?
Sterile technique is a procedure that includes the medical and the laboratory techniques like with cultures. Sterile technique includes techniques such as flame sterilization and it should be used in hospital operating theatres.
Why flame is observed during the burning of a substance?
During combustion, energy is released. Some of this energy takes the form of light, making the flame visible.
Does the Olympic Flame burn during the Olympic Games?
no
The bottom of a test tube was covered with soot after an experiment which required heating. What kind of flame was used during the experiment?
A luminous flame
How would fireman and meatloaf be written using the kenning technique?
Fireman could be written as "flame-tamer" and meatloaf could be written as "feast-cake."
Why was a flame used during the Ancient Olympics?
Because of king zeues
Glowing gases observed during combustion?
Glowing gases observed during combustion is called a "flame."
Application of flame photometry in biological samples?
this technique is used for the determination of sodium potassium and lithium (in case of Lithium therapy) in biological specimens received in laboratories
