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Why is it important to cut a DNA as closely to the desired gene as possible?
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What is the role of Cloning Vector in recombinant DNA technology?
The desired gene is cut from its original DNA by using restriction enzyme. The vector (plasmid) is also cut with the same restriction enzyme so that both the vector and the desired gene have the same sticky end. They are bonded together by DNA ligase. Why desired gene are cloned into vector? This is because vector have the ability to reproduce by itself and at the same time carry the desired gene together; in another word, it can replicated the desired gene in the host cell.
Which of the following attaches the target gene to a desired location?
DNA ligase
When plasmids are used to produce a desired protein what happens?
the desired gene is inserted into the plasmid and the plasmid is returned to the bacterium by transformation.
When genes move between two closely associates specie's what is this?
Gene flow.
How is the type of cell into which a new gene is inserted important in gene therapy?
a spelling
Which outcome is possible using genetic engineering but not using selective breeding?
The insertion of a [desired] gene segment into an existing chromosome.
A general scheme for genetic engineering is?
obtain DNA with the desired gene, cut the DNA into pieces, copy the pieces of DNA, identify copies of the desired gene, and transfer the desired gene to the recipient organism. for Plato the answer is A. not B. C. or D.
What is the role of Cloning Vector in recombinant DNA technology?
The desired gene is cut from its original DNA by using restriction enzyme. The vector (plasmid) is also cut with the same restriction enzyme so that both the vector and the desired gene have the same sticky end. They are bonded together by DNA ligase. Why desired gene are cloned into vector? This is because vector have the ability to reproduce by itself and at the same time carry the desired gene together; in another word, it can replicated the desired gene in the host cell.
What is the desired outcome of gene therapy?
Gene therapy has the desired outcome of eradicating or curing diseases that are caused by a specific abnormality or mutation of a gene that is a killer. It is a developing science and slowly getting results.
What is the desired result of gene therapy?
When the replaced genes functions properly and corrects the defect in that region a gene therapy is successful and the desired result is achieved.
Which of the following attaches the target gene to a desired location?
DNA ligase
When plasmids are used to produce a desired protein what happens?
the desired gene is inserted into the plasmid and the plasmid is returned to the bacterium by transformation.
When genes move between two closely associates specie's what is this?
Gene flow.
What is the definition of gene shuffling?
Gene shuffling is the process of recombining the starting pool of sequences to generate new gene-sequences that subsequently can be screened for particular desired characteristics.
Can a single gene control a sigle trait?
Its possible. A single gene defines a single protein.
How is the type of cell into which a new gene is inserted important in gene therapy?
a spelling
What is transient expression?
Transient expression is a temporary expression of a gene or protein in a cell or organism, typically achieved using vectors or systems that do not integrate the DNA into the host genome. This allows for rapid and high-level production of the desired product without permanently modifying the host cell's genome. Transient expression is often used for research purposes or for producing recombinant proteins.
