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because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.

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13y ago
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15y ago

The heat sterilises the loop of any previous microbes on it, so they will not interfere with the experiment or affect the outcome.

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10y ago

To kill any bacteria on it.

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12y ago

in order to sterlize the loop

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Q: Why is it necessary to flame the loop between cultures?
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Why must it e flamed after making an inoculation?

We use to flame the inoculating loop after inoculation because during inoculation many bacterial cell get attached to loop which can further contaminate the inoculation of other cells so to destroy the previous sticked celled it is necessary to flame burn the loop


How can a contamined wire loop be cleaned?

insert the loop into the burner flame until all of the salt has been burned off


Why must pay attention to how flame loop?

because it can burn you or the microorganism will not be killed


What is the purpose of flaming the loop after use?

I'm assuming you mean an "innoculating loop" in microbiology. You flame the loop to kill the microoganisms on the loop before using it again to prevent mixing different bacterial colonies and contaminating them.


How would you make sure a inoculating loop was sterile?

Pass the loop through a flame 2 or 3 times for about 10 seconds at a time.


What is the hazard of the splattering tendency in sterilizing a wire loop over a flame?

Heat mat


What is the purpose of flaming the loop before and after use?

Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.


How do you conduct a flame test?

Bunsen burner Piece of wire with a loop on the end Colour chart Undergraduate student Fire extinguisher (just to be safe)


How will you know when you have flamed the inoculating loop or needle long enough?

when we show the flame in the loop ,for 1 minute its gets brownish colur


Why are inoculating loop and needle flamed before and after use?

The heat from the flame destroys any bacteria on the wire loop. This prevents any transfer or cross contamination by unwanted or unintended bacteria. Put simply, this action thoroughly cleans the innoculating loop. To correctly perform this action, the loop is passed UP the column of flame from the bottom, where it is (relatively) cooler, to the TOP where the fully oxygenated gas is at its hottest temperature. Allow the loop to glow red hot for a few (2-3) seconds and then remove it from the flame. Allow a few seconds for the air to cool the loop, and it is again ready for its next use. Note: Small sparks may be seen to erupt as any bacterial cells burst into flame.


Why must you not flame your loop then quickly try to pick up bacteria with it?

The heat can kill the bacteria


What are the names of the puffles on elite puffle foce on club penguin?

loop bouncer blast flame flit