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Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.
500 ml
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
To make a 9 grams per liter solution of NaCl, simply dissolve 9 grams of NaCl in enough water to make a total volume of 1 liter. Stir or shake the solution until the NaCl is completely dissolved. Measure the volume accurately using a graduated cylinder or volumetric flask.
Louis broiled broth for 15 min. He then let it sit for one year in a swan neck flask. He noticed that some dust had accumulated in the top part of the flask. So he took a sample, form the broth inside the flask. He noticed that nothing was inside of growing in it. So he then preceded to shake the broth in the flask in such a way, that it would touch the dust at the top. He then took a sample from that and it was highly bacteria ridden.
Shake flasks, such as the Erlenmeyer flask, are designed to prevent splashing. They promote oxygen transfer and prevent vibration, allowing for successful fermentation.
Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.
500 ml
so that the solutions mix properly
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
It is necessary to shake an oil and vinegar salad dressing before adding it to a salad because they separate easily.
0.1M is 1/10 molar whereas 1mM is 1 millimolar and thus 1/1000 molar. There is thus a 1:100 dilution. So 10:1000 would be the same. To a 1000ml volumetric flask, pipete 10mls of 0.1M EDTA solution. Make up to the mark with deionized water. Mix and shake and you will have 1000mls of 1mM EDTA solution.
To make a 9 grams per liter solution of NaCl, simply dissolve 9 grams of NaCl in enough water to make a total volume of 1 liter. Stir or shake the solution until the NaCl is completely dissolved. Measure the volume accurately using a graduated cylinder or volumetric flask.
No, but it will help
Because when it settles the oil in it separates and sits in the bottom so you have to shake it to emulsify it again.
You get meth in about 40 minutes. Throw it all on one flask and let it sit for a while shake the flask it if starts to bubble up too much. Then when it calms down heat it to about 180f until it doesnt look like anything else is happening.
Shake it firmly, then change ends and repeat, as necessary.