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Why must a straight inoculating needle be used when inoculating an agar deep tube?
Wiki User
∙ 13y ago
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
Wiki User
∙ 13y ago
Wiki User
∙ 13y agobcoz we obtained small amount culture for pursuing our practical without touching the side of test tubes......
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Inoculating an agar deep tube?
inoculation needle
What are the uses of deep agar?
Agar deeps are used to see whether an organism requires oxygen to grow. If there is spreading growth only at the bottom of the tube, the organism is an obligate anaerobe (meaning it cannot tolerate oxygen). If there is growth only at the surface of the agar, the organism is an obligate aerobe (it cannot grow without oxygen). And if there is growth all along the point of innoculation, the organism is a facultative anaerobe and can survive either way.
What are the advantages and disadvantages of using agar deep for culture media?
You shouldn't use too much, only a small layer is required, any more is just wasteful.
What type of roots grow straight down deep into the groun?
thorns and stinging hairs
Did growth happen at a different level in agar deep?
The agar deep stab is an experiment to determine the affect that oxygen has on a culture. If growth is found only at the top, this indicates an obligate aerobe. If growth is found at the bottom only, then it would be a obligate anaerobe. If growth is found all throughout the stab, this would indicate a facultative anaerobe. Obligate aerobes require oxygen to survive. Obligate anaerobes cannot survive with oxygen present. Facultative anaerobes can survive in both cases but usually prefer aerobic conditions.
Inoculating an agar deep tube?
inoculation needle
Why does a straight inoculating needle used to inoculate an agar deep tube?
because you want to introduces as little air as possible
When inoculating agar deep stabs why do you have to insert and remove the needle along the same stab line?
To minimize the introduction of unwanted oxygen into the medium.
Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.
What is slant broth and deep?
a slant broth is a type of inoculating agar to gather bacterial data from.
Why did you stab the peptone iron agar instead of inoculating only the surface?
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
How can anaerobes be maintained in pure cultures?
Anarobes can be inoculated in an agar slant using an inoculating needle. The needle is able to put the anarobes deep inside the agar. The anarobes can then grow in the anarobic environment provided. The agar slant should then be refrigerated after suitable growth is achieved.
What is the advantage of an agar slant versus an agar deep?
agar is botained from a red alga called gelidium
What is agar deep?
Agar deep refers to a measure used with microbes. It is a measurement of how much gas a microbe requires for survival.
Why important to use Agar Deep Stab medium soon after preparation?
Over time, free oxygen will diffuse into the agar.
What are the uses of deep agar?
Agar deeps are used to see whether an organism requires oxygen to grow. If there is spreading growth only at the bottom of the tube, the organism is an obligate anaerobe (meaning it cannot tolerate oxygen). If there is growth only at the surface of the agar, the organism is an obligate aerobe (it cannot grow without oxygen). And if there is growth all along the point of innoculation, the organism is a facultative anaerobe and can survive either way.
What is the first step you should take after a needlestick?
My step brother kneeled down on a needle once, It was a big needle that went about 2 inches into his knee. I think that if you get a needle in your skin and its not deep then just pull it out yourself. If its deep and you think you need medical attention then go straight to the local emergency room or contact your doctor immediately!
