Slight heating helps in fixing the cells on to the surface of the glass slide
You will cook it!
It dries the smear and fixes the cells to the slide
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
smear will be washed( no smear will be left on the slide)
In order to heat fix your microbe to your slide - you need to let your smear air dry. Once it is completely air dried. Pass the slide 2 or 3 times quickly over an open flame like a Bunsen burner. This does three things, 1. Kills the bacteria 2. Firmly affixes the smear to the microscope slide. 3. Allows the sample to more readily take up the stain.
You will cook it!
It dries the smear and fixes the cells to the slide
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Smear is one of the forms in which organisms are viwed under the light microscope in microbiology, the other form is as wet mounts, remember that smear is used to view killed organisms the steps in making smear are as follow: 1-Get a grease free slide and a wire loop. 2-sterilize the wire loop by flaming. 3-deep the wire loop in a normal saline. 4-touch the slide with the wire loop, there will be a drop of normal saline on the slide. 4-sterilize the wire loop again and touch a small part of a colony in petri dish, then mix it with the normal saline on the slide. 5-allow it to air dry. 6-pass it through an open flame three times(heat fixation). Then view under the microscopes. The heat fixation is done in order to: 1-to fix the smear to the slide. 2-to kill the organism 3-to alter the organism so that it can accept stain readily.
Heat fixing a smear kills the bacteria with minimal distortion, allows for better staining, and firmly affixes the bacteria to the slide. Chemical fixing is used to preserve fine cellular structures and might stop internal processes in place, protect the cell from damage, or strengthen the cell's structure.
The slide can get damaged.
The smear will not adhere well if there is grease on the slide.
To avoid denaturing and destroying the smear.
smear will be washed( no smear will be left on the slide)
In order to heat fix your microbe to your slide - you need to let your smear air dry. Once it is completely air dried. Pass the slide 2 or 3 times quickly over an open flame like a Bunsen burner. This does three things, 1. Kills the bacteria 2. Firmly affixes the smear to the microscope slide. 3. Allows the sample to more readily take up the stain.
The smear will not adhere well if there is grease on the slide.
Another name for a slide that has no liquid or cover slip is a dry smear or a dry mount. It is usually used to examine blood.