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Why should the concentration of sample be low in spectrophotometer readings?
Suobo
When doing reading on a spectrophotometer, the sample being studied is either a color change or a precipitated compound, depending on the wavelength that it is being read. If it is a precipitated compound and it has a very high concentration, then you run the risk of the light being used to measure the absorbance not going through. In which case you have total absorbance but it is inaccurate in helping you determine the concentration of your sample because you are unsure where the concentration limit is for that wavelength, and your sample could possibly be able to absorb more. In which case you still can't calculate the concentration of the sample.
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∙ 16y ago
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What condition should use the standard addition method?
The standard addition would be used when determining the concentration of a sample because of matrix effect problems (matrix effect occurs when unknown sample contains many impurities).
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