Concentration of sample= estimated LOQ concentration (µg/mL) x 1/desired LOQ (%) x 100LOQ should be Equal to or less than 0.05% of that of test concentration. Response of the impurity should be NLT 2000 at LOQ level for better precision.
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
mixture of enantiomers can be separated by HPLC
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
HPLC is used to analyze, identify, purify and quantify organic compounds.
"RS-HPLC method" means "Related Substance HPLC Method".
concentration of buffer effects in buffer strength
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
whats wrong with it?
HPLC is advanced
Whether GC or HPLC is a more preferable analytical method depends on the material being analyzed for, the likely concentration in which it is found, and what medium it may be in/on (water, oil, different kinds of filters, etc.) There is no single, universally applicable answer.
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
why RT was shifting & how to RT calculation in HPLC
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
mixture of enantiomers can be separated by HPLC
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.