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Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Do you apply waterproofing product before or after staining the deck?
After. If you waterproof before staining, the stain won't make it to the wood to stain it.
What is staining rack?
Staining rack is used to hold many glass slides at a time. By putting slides on a staining rack, you can pour dye simultaneously and it will help to reduce extra dye or stain.
What is an orthochromic stain?
An orthachromic stain is used in microscopy. It remains the same colour regardless of what it is staining, unlike metachromic stains, that change colour.
What are the advantages of the simple stain technique over the negative stain technique?
Negative staining has a dark contrasted background and the bacteria is white. Simple staining has a white background and bacteria is the color depended on your stain color.Negative staining when prepared is NOT heat fixed but simple staining when prepared is heat fixed. Heat fixed means when preparing slide with bacteria on it, it is passed over some type of flame, like a Bunsen burner flame, three times or four times.
What is secondary stain?
A secondary stain is Methylene blue. This type of stain is used in a acid fast staining. This type of staining test can determine medical conditions such as tuberculosis.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
What bacterial staining technique is used when water is used to remove excess stain from the slide?
simple
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Concrete Staining?
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Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
Do you apply waterproofing product before or after staining the deck?
After. If you waterproof before staining, the stain won't make it to the wood to stain it.
Why safranine and not methylene blue is used to stain plant cells?
Both are used in staining but for different purposes .
What is staining rack?
Staining rack is used to hold many glass slides at a time. By putting slides on a staining rack, you can pour dye simultaneously and it will help to reduce extra dye or stain.
