Serial dilution technique
WIDAL
It's to see if you have VD.
Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
Both force you to read your lab manual.
That is a good question! The answer is simple. You do step by step dilution of the serum with probably normal (isotonic) saline solution and test the same for Widal test. This is how you do quantitative test for many other tests also.
What is false positive in widal test
widal slide is easier and it save time but widal tube test is not easy and it takes time.
I mean widal test result positive after treatment
Isolated Widal test reading does not have much clinical significance. Rising titre of Widal test done one week apart, in a febrile patient, indicates typhoid fever. You can have such rising titre of Widal test in healthy patient, after dose of typhoid vaccine.
Serial dilution technique
1:80 titre is significant for determination of widal positive.
WIDAL
widal test
To a certain extent yes.The post antibiotics Widal Test will have Low titers of agglutinins.Hence the test may be reported as False Negative.
widal test.
manual test of widal method