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the microorganism required carbon source and use for the production of energy
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Composition of potato dextrose agar media?
Potato Dextrose Agar (PDA) - a relatively rich medium for growing a wide range of fungi. - use for isolation and enumeration of yeasts and molds from dairy and other food products. - stimulation of conidia production in fungi. useful in slide cultures also demonstrate pigment production of T. rubrum
Why would you use a UV lamp for microbiology?
As UV only kill the Microorganism.Microbiological media contain the agar & for agar dissolve at 90 degree temperature that's why we cannot use UV to sterlize microbiological media
What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?
Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.
Who is Fannie Eilshemius Hesse?
The wife of Walther Hesse, collaborator of Koch. She suggest to his husband the use of agar in order to solidify media for microbial culture.
What agar gel is used to culture germs?
Agar is a non-nutritive substrate to which nutrients are added . It is used as a solidifying agent in bacterial culture media. Without agar, the medium is liquid while when agar is included, the medium becomes solid (gel-like) soon after cooling.Unlike liquid media, solid media give the opportunity that bacteria can grow as separated colonies. This helps to identify bacteria because different types of bacteria have different types of colony morphology (shape, color, size, etc).The first step to check if a specific type of bacteria is present in a specimen is to separate this bacteria from the other types of bacteria present . This is accomplished by cultivating a small portion of the specimen on solid media to give separated colonies.There are many different types of agar containing media. The most common is nutrient agar as it is considered the least selective and most microbes can grow on it, it is made with beef broth. However, microbiologists will try many things to get fussy bacteria to grow including extract of armadillo! Some media are designed to only allow the growth of certain bacterial species to help microbiologist narrow down their search. These are called selective media. Some media are designed with colour change tests in them so as to allow the further narrowing down of the species of bacteria. These are called differential media.
What specific medium must be use in testing the effectiveness of antibiotics?
A growth medium must be used. The most common is Mueller-Hinton agar, but potato dextrose agar or other growth media could also be used.
Composition of potato dextrose agar media?
Potato Dextrose Agar (PDA) - a relatively rich medium for growing a wide range of fungi. - use for isolation and enumeration of yeasts and molds from dairy and other food products. - stimulation of conidia production in fungi. useful in slide cultures also demonstrate pigment production of T. rubrum
Why would you use a UV lamp for microbiology?
As UV only kill the Microorganism.Microbiological media contain the agar & for agar dissolve at 90 degree temperature that's why we cannot use UV to sterlize microbiological media
How do you recognize bacteria on agar plate and not confusing bacteria with eukaryotes like yeast or fungi?
Use selective media agar plates. Different types of agar will let bacteria grow and inhibit fungal growth, or vice versa.
What is the use of luria bertani media?
why is meuller hinton(MH) agar specially used for antibiotic succeptibility testing?
What is non selective media?
Non-selective is when we use agar for bacterial growth in general. Mean not to differentiate between them but just to see their colonies and growth. TSA and Chocolate agar are nonselective.
What is the use of sabouraud's dextrose agar and the role of pH in this selectivity?
Selects for yeasts, and a pH indicator should differentiate whether they produce acid as byproducts, thereby further helping one determine what type of yeast it is.
Why shouldn't you dig into agar with the loop?
Streaking is to produce single colonies. If we are digging to the agar while streaking the microbes, instead of growing on the agar surface grows in the subsurface as well. These colonies may be difficult to isolate.
What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?
Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.
Who is Fannie Eilshemius Hesse?
The wife of Walther Hesse, collaborator of Koch. She suggest to his husband the use of agar in order to solidify media for microbial culture.
What agar gel is used to culture germs?
Agar is a non-nutritive substrate to which nutrients are added . It is used as a solidifying agent in bacterial culture media. Without agar, the medium is liquid while when agar is included, the medium becomes solid (gel-like) soon after cooling.Unlike liquid media, solid media give the opportunity that bacteria can grow as separated colonies. This helps to identify bacteria because different types of bacteria have different types of colony morphology (shape, color, size, etc).The first step to check if a specific type of bacteria is present in a specimen is to separate this bacteria from the other types of bacteria present . This is accomplished by cultivating a small portion of the specimen on solid media to give separated colonies.There are many different types of agar containing media. The most common is nutrient agar as it is considered the least selective and most microbes can grow on it, it is made with beef broth. However, microbiologists will try many things to get fussy bacteria to grow including extract of armadillo! Some media are designed to only allow the growth of certain bacterial species to help microbiologist narrow down their search. These are called selective media. Some media are designed with colour change tests in them so as to allow the further narrowing down of the species of bacteria. These are called differential media.
What is another word for glucose when it is man made for IV use?
Dextrose is used to describe glucose when manmade for IV use. D5W is an abbreviation for 5% dextrose in water, a common IV solution.
