0
Want this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
What is the role of protinase K in DNA isolation?
I believe the role of proteinase K in a DNA isolation is just to digest proteins. Proteinase K is a protein digesting enzyme. Digesting proteins is important in a DNA isolation because the proteins included in your DNA before you treat it with proK likely include some DNAses. If you didn't use proK, your DNA would degrade very quickly.
What is the function of Proteinase K in DNA extraction?
Proteinase K is most often used in extracting DNA from while blood cells (or leukocytes). The first step in DNA isolation is to break open the cell and release the cytoplasmic contents, which includes the nucleus, in which we find DNA. Proteinase K is a protease (an enzyme capable of digesting proteins). It is used to digest the cell surface proteins. When cell surface proteins are digested, the integrity of the cell membrane is compromised leading to cell lysis (or the breaking open of the cell)
What is feldspar's chemical formula?
x Al(Al,Si)3O8, where x can be sodium (Na) and/or calcium (Ca) and/or potassium (K).
How do you use constant K?
In chemistry, K is 273+Degrees C.
What is hydrothermal leaching of rocks?
crustal Rocks that consists of Ca,Al,Si, Na, K, Mg, Fe and O.
How do you prepare 50mM TES buffer?
In order to prepare 50mM TES buffer, you will need to add in approximately 1000 ml of Proteinase K solution. From there, you will need to separate and stack the gels.
What happens if you add too much proteinase k to a lysis buffer when performing DNA extraction?
it depends what "too much" is. a concentration up to 1mg/mL or slightly higher can be still ok. proteinase K should digest only proteins, leaving your DNA intact.
What is the role of protinase K in DNA isolation?
I believe the role of proteinase K in a DNA isolation is just to digest proteins. Proteinase K is a protein digesting enzyme. Digesting proteins is important in a DNA isolation because the proteins included in your DNA before you treat it with proK likely include some DNAses. If you didn't use proK, your DNA would degrade very quickly.
What happens if you add Proteinase K more than you need?
it lalala lopsie
What is the function of Proteinase K in DNA extraction?
Proteinase K is most often used in extracting DNA from while blood cells (or leukocytes). The first step in DNA isolation is to break open the cell and release the cytoplasmic contents, which includes the nucleus, in which we find DNA. Proteinase K is a protease (an enzyme capable of digesting proteins). It is used to digest the cell surface proteins. When cell surface proteins are digested, the integrity of the cell membrane is compromised leading to cell lysis (or the breaking open of the cell)
What is the quickest most effective way to inactivate Proteinase K?
As with most protein enzymes change the temperature or change the pH significantly.
What has the author M K Al-Halabi written?
M. K. Al-Halabi has written: 'Bearing capacity of sand'
What has the author M K Shah Al-Azhari written?
M. K. Shah Al-Azhari has written: 'Maqalat'
Write a Program to implement error correction using hamming code?
/* WRITTEN BY : BIBHAKAR JHA OBJECTIVE: TO IMPLEMENT HAMMING CODE IN C */ // PROGRAM CODE : #include<stdio.h> #include<conio.h> #include<string.h> #include<math.h> void main() { char sender[20],buffer[50],reciver[20]; int d,p=0,i=0,j,k,length,power,count; clrscr(); printf("ENTER THE MESSAGE IN BIT FORM:- "); gets(sender); d=strlen(sender); //power for(p=0; ;p++) { if(pow(2,p)>=d+p) { break; } } p--; /* COPY THE BITS INTO BUFFER */ i=1; power=0; j=0; do { if(i==pow(2,power)) { buffer[i]='0'; power++; i++; } else { buffer[i]=sender[j]; j++; i++; } }while(j<=d); buffer[i]='\0'; //APPLY BIT IN CODE count=0; length=d+p; j=0; for(j=0;j<=p;j++) { k=pow(2,j); for(i=k+1;i<=length; ) { if(k==1) { if(buffer[i]=='1') { count++; } i=i+2; } else { if(buffer[i]=='1') { count++; } i++; if(i%k==0) { i=i+k; } } } if(count%2==1) { buffer[k]='1'; } count=0; } printf(" ENCODED MESSAGE IS \n "); i=1; do { printf("%c",buffer[i]); i++; }while(buffer[i]!='\0'); getch(); }
What has the author Muhammad K Al-Atawneh written?
Muhammad K. Al-Atawneh has written: 'Wahhabi Islam facing the challenges of modernity'
Largest atomic radius out of Ca Na Fe Al and k?
K
In Final Fantasy X where is the Al Bhed letter K found?
The Al Bhed letter K, which translates to G, is in the Luca Sphere Theater. The Al Bhed letter G, which translates to K, is on the Djose Highroad. I wasn't sure which one you meant.
