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how to make a well on the agar for pour the extract
The gel in a Petri dish is called "Agar".
Agar
The color of salmonella in bismuth sulphite agar is brown to black color with metallic sheen.
Because the agar remains a solid anywhere below 42 degrees Celsius. A typical environment is never going to raise above 42 degrees therefore it will always remain solid even in the human body (37 degrees Celsius). Having that said, most microorganisms cannot digest agar unless it is liquid.
Depending on the media agar solidifies at 32 to 40 degrees Celsius. It remains firm up to about 65 degree Celsius.
85C
Agar, which is an extract of red marine algae, is preferred because only a few microbes can degrade the agar. It will remain as a solid during the growth of bacteria. It will stay as a liquid until cooled to 40 degrees Celsius. Once a solid, it will stay solid unless incubated at temperatures over 85 degrees Celsius. Agar melts at 85 °C (358 K, 185 °F) and will solidify from 32-40 °C (305-313 K, 90-104 °F). Since many scientific applications require incubation at temperatures close to human body temperature (37 °C), agar is more appropriate than other solidifying agents that melt at this temperature, such as Gelatin.
Agar, which is an extract of red marine algae, is preferred because only a few microbes can degrade the agar. It will remain as a solid during the growth of bacteria. It will stay as a liquid until cooled to 40 degrees Celsius. Once a solid, it will stay solid unless incubated at temperatures over 85 degrees Celsius. Agar melts at 85 °C (358 K, 185 °F) and will solidify from 32-40 °C (305-313 K, 90-104 °F). Since many scientific applications require incubation at temperatures close to human body temperature (37 °C), agar is more appropriate than other solidifying agents that melt at this temperature, such as Gelatin.
In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.
Most bacteria grows within that range of temperature. 74°C will kill almost all bacteria. Bacteria were among the first life forms to appear on Earth, and are present in most of its habitats.
Agar, which is an extract of red marine algae, is preferred because only a few microbes can degrade the agar. It will remain as a solid during the growth of bacteria. It will stay as a liquid until cooled to 40 degrees Celsius. Once a solid, it will stay solid unless incubated at temperatures over 85 degrees Celsius. Agar melts at 85 °C (358 K, 185 °F) and will solidify from 32-40 °C (305-313 K, 90-104 °F). Since many scientific applications require incubation at temperatures close to human body temperature (37 °C), agar is more appropriate than other solidifying agents that melt at this temperature, such as Gelatin.
Agar comes from Red Algae (primarily the Gracilaria genus).It mainly is produed from the red algae Gracilaria lichenoides.This algae is harvested along the western coast of the United States and in parts of Asia. To extract the agar the Algae is boiled, cooled, purified and then dried. The agar is then make into blocks, flakes, or granules.("Agar agar" is usually abbreviated as "agar".)
Solid medium
a solid medium
im stuck on the same thing!