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Generally, if you increase a reagent and there is no increase in the reaction, then it is limited by the amount of another reagent present. For example, a mole of Sodium Hydrogen Carbonate will react with a mole of Acetic Acid. However, doubling the Acetic acid will not result in a greater reaction because it is limited by the amount of Sodium Hydrogen Carbonate present.

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Q: Why was there no increase in reaction with 8g compared to 4g of substrate?
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