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Why would bacteria not grow on an agar plate treated with a disinfectant?
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What Carbon source would be available to a soil bacteria on a R2A plate?
Dextrose
Describe the aseptic technique that would be used when flooding the agar plate with bacteria?
An agar plate was flooded with a culture of a species of bacterium usually found in the mouth. Four steriled paper discs, A, B, C, and D, each containing a different brand of mouthwash, were then placed on the agar plate. The drawing shows the appearance of the plate after it had been incubated below body temperature for three days, this is to ensure that the bacteria are not harmful to humans. Describe the aseptic technique that would be used when flooding the agar plate with bacteria
What are some limitations of a phenol coefficient test?
Your results might be different then these but I hope this will give you an idea for an answera. In our lab we observed growth in all of the test tubes. Originally we had hypothesized that the tubes with higher concentrations of disinfectant would inhibit the growth of the inoculated organism. This experiment allows us to conclude that the disinfectant is not very efficient. Although we were able to come to a conclusion, our results are limited to the organisms that we used to inoculate the test tubes. Different organisms might be more resistant to the disinfectant. A conclusion that the disinfectant we used is inefficient with all bacteria cannot be made.
What happens when tap water is treated with alum?
the dirts and bacteria in the water would coagolate or clump together~it is also a method of purifying water..
What are the surface colonies on a pour plate larger than those within the medium?
The surface colonies on a pour plate larger than those within the medium especially aerobic bacteria within the medium would be a restriction of growth. The restriction of growth would be due to the lack of oxygen.
What does it mean to inoculate an agar plate?
to inoculate an agar plate you would place bacteria on the agar to grow
How would you change the bacterias plate to best tell if they are ampicillin resistant?
The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.
What Carbon source would be available to a soil bacteria on a R2A plate?
Dextrose
Why nitrogen is used as disinfectant?
Nitrogen is NOT a disinfectant. The air we breath is 80% Nitrogen, if Nitrogen were a disinfectant there would be no life on Earth!
What would you need to do to determine whether bacteria growing on a petri plate are anaerobes?
some sense
Describe the aseptic technique that would be used when flooding the agar plate with bacteria?
An agar plate was flooded with a culture of a species of bacterium usually found in the mouth. Four steriled paper discs, A, B, C, and D, each containing a different brand of mouthwash, were then placed on the agar plate. The drawing shows the appearance of the plate after it had been incubated below body temperature for three days, this is to ensure that the bacteria are not harmful to humans. Describe the aseptic technique that would be used when flooding the agar plate with bacteria
What would act as a disinfectant?
warm water
What one plate would you first inspect to conclude that the transformation occurred successfully Why?
If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got the antibiotic resistance gene on the plasmid and the transformation was unsuccessful. If some colonies are found, that means some bacteria contain the plamis containing the antibiotic resistance gene and those colonies can the transformation was successful.
What type of cardiovascular diseases may be treated with antibiotics?
Pericarditis, Endocarditis, and Myocarditis. All of these are inflammation diseases of the heart and if it is caused by bacteria, then it can be treated by antibiotics, because antibiotics clear up infections/bacteria.
What are some limitations of a phenol coefficient test?
Your results might be different then these but I hope this will give you an idea for an answera. In our lab we observed growth in all of the test tubes. Originally we had hypothesized that the tubes with higher concentrations of disinfectant would inhibit the growth of the inoculated organism. This experiment allows us to conclude that the disinfectant is not very efficient. Although we were able to come to a conclusion, our results are limited to the organisms that we used to inoculate the test tubes. Different organisms might be more resistant to the disinfectant. A conclusion that the disinfectant we used is inefficient with all bacteria cannot be made.
What happens when tap water is treated with alum?
the dirts and bacteria in the water would coagolate or clump together~it is also a method of purifying water..
Advantages of pour plate method over other methods of bacterial colony?
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
