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What tool does one use to separate DNA fragments?
a Polyacrylamide gel
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
What is the principle involved in the isolation procedure?
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
Why use chloroform in DNA isolation?
DNA is soluble in chloroform more than water. So we use it.
What are the scientific uses for polyacrylamide?
it is used in gel electrophoresis.....for the separation of DNA fragments
what is the gel in Gel Electrophoresis?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
Why you use LiCl in plasmid isolation by telt method?
helps in DNA ppt
Can you use spirit for DNA precipitation?
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
What is the use of sarkosyl in DNA isolation?
it is SDS like detergent added at to lyse cells
What does ctab use for isolation of DNA from animal blood?
CTAB is a detergent used to denature proteins from samples. Once the protens have been denatures, the isolation of DNA from the non required waste materials can begin.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
