Since the field of view is a circle, the size of the field of view is it's area. You would need to find the diameter of the field of view, using a transparent ruler or a micrometer. Divide the diameter measurement by 2 to get the radius. Then use the formula for the area of a circle, Area = πr2. For example, you measure the diameter of the field of view to be 2.14mm. Divide 2.14mm by 2 to get the radius, and you get 1.07mm. Square 1.07mm, which is 1.14mm2. Multiply x 3.14 (pi), and you get 3.58mm2. So the field of view for this example would be 3.58mm2.The field of view differs with different magnifications. The lower the magnification, the larger the field of view.
40X.
A microscope lens with a power of 40X will magnify an object 40 times its actual size.
As the diameter of the iris diaphragm increases, the depth of field decreases. A larger diaphragm lets in more light, leading to a shallower depth of field with less of the image in focus. Conversely, a smaller diaphragm increases depth of field by letting in less light and making more of the image appear sharp.
40x
The field of view's diameter is inversely proportional to magnification; thus, the 5.6mm diameter at 40x magnification would become 140mm at 1x magnification. Mathematically, Field of view diameter = FOV1 / Magnification1 = FOV2 / Magnification2.
As you change objectives from 4x to 10x to 40x, the field of view will decrease. This is because higher magnification objectives bring smaller areas into focus, resulting in a narrower field of view.
To find the new field of view at 400X magnification, you would divide the original field of view by the magnification increase factor (which is 10 in this case since you are going from 40X to 400X). So, 6000 um / 10 = 600 um. Therefore, the field of view at 400X magnification would be 600 micrometers.
To measure the length and width of a paramecium in microns using a 40x magnification microscope, you can use a calibrated eyepiece graticule or stage micrometer to determine the field of view in microns at that magnification. Then, you can measure the paramecium by counting the number of microns it spans across using the field of view as a reference. This will allow you to estimate the length and width of the paramecium in microns.
10X
Field diameter is calculated by measuring the distance across the field of view of a microscope, then dividing that measurement by the magnification of the objective lens being used. This gives you the field diameter in micrometers.
The field of view would be 80 times the diameter.
The equation goes like this and works for both medium AND high feild diameter : Medium(High) DIA. = Low Diameter / [Med(High)mag/low mag] Brackets () are NOT for multiplication, they are for the other formula.
You can estimate the size of the object by comparing the field diameters observed under the low power objective lens (4x) and high power objective lens (40x). Calculate the ratio of the field diameters (40x/4x = 10), and use this ratio to estimate the size of the object viewed under the high power objective lens. Simply multiply the size of the object viewed under the low power objective lens by the ratio (field diameter at 4x) to get an estimation.
Magnification is inversely proportional to the diameter of the field of view.
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400x gives the smallest field of view. The magnification of the instrument, and the field of view are inversely rational.