If it has a use by date you are supposed to observe that date. I personally would ignore the use by date and use it
because methylene blue turns colourless when it is reduced by hydrogen. during respiration hydrogen is produced and instead of reducing NAD, it reduces methylene blue and turns methylene blue colourless. if methylene blue goes from blue to colourless then this shows that the cell is respiring as it is producing a suffiecient amount of hydrogen to decolourise methylene blue
When you added methylene blue to the living yeast cell, the methylene blue is actually a dye so you could see the cells, therefore, the yeast cell became blue. It is much the same with hair dye. If you have a bottle of blue hair dye, it will dye your hair blue.
Methylene blue is necessary for various applications, such as in medical diagnostics, staining biological tissues, and as a medication to treat methemoglobinemia. It is also used as a redox indicator in chemical reactions and as a dye in various industries. In microscopy, methylene blue can help highlight cell structures for better visualization.
Methylene blue can act on only dead yeast cells because it is able to penetrate the cell membrane easier when the cell is dead, allowing it to enter and stain the cell. In live yeast cells, the cell membrane is intact and acts as a barrier, preventing methylene blue from entering and staining the cell.
Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable. Also used to staining the blood film and used in cytology. It gives a blue coloration to the human cheek cell.
If methylene blue is blue, it means that the compound is in its oxidized state (methylene blue) and has accepted electrons. Methylene blue can exist in both oxidized (blue) and reduced (colorless) forms depending on its redox state.
i think the methylene blue will be make aqua blue because the charcoal will penerate the color of methylene blue,,,there are absorption process,,,in the charoal between the methylene blue.... (kharlz)
Methylene blue stains everything blue.
because methylene blue turns colourless when it is reduced by hydrogen. during respiration hydrogen is produced and instead of reducing NAD, it reduces methylene blue and turns methylene blue colourless. if methylene blue goes from blue to colourless then this shows that the cell is respiring as it is producing a suffiecient amount of hydrogen to decolourise methylene blue
Methylene blue will diffuse faster than potassium permanganate. Methylene blue has a smaller molecular size and a higher diffusion rate compared to potassium permanganate.
Methylene blue appears blue because of its molecular structure, which allows it to absorb light in the red-orange range of the visible spectrum and reflect or transmit light in the blue range. This selective absorption and reflection of light wavelengths give methylene blue its distinctive blue color.
NADH
0.05%
Potassium permanganate has a higher molecular weight compared to methylene blue. The molecular weight of potassium permanganate is approximately 158.03 g/mol while the molecular weight of methylene blue is around 319.85 g/mol.
Methylene blue is necessary for one thing. It is what helps transports cells.
you can get methylene blue powder from a scientific store, it comes in powdered form. its pretty soluble in water and alcohol etc. the stain is made by dissolving an appropriate amount on methylene blue in a solvent, e.g for 0.1 dissolve 0.1% gram of methylene blue in 100 gram water, for 9% dissolve 9 grams
Methylene blue would enhance your observation more than iodide staining. Methylene blue binds to nucleic acids and proteins, making them more visible under a microscope. Iodide staining is primarily used for visualizing lipids in samples such as plant tissues.