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Haemophilus influenzae is a species of bacteria that is the source of the HindIII restriction enzyme that cleaves the palindromic DNA sequence 5'-AAGCTT-3' in the presence of the cofactor Mg2+ via hydrolysis. While restriction enzymes cleave at specific DNA sequences, they are first required to bind non-specifically with the DNA backbone before localizing to the restriction site. On average, the restriction enzyme will form 15-20 hydrogen bonds with the bases of the recognition sequence. With the aid of other Van der Waals interactions, this bonding facilitates a conformational change of the DNA-enzyme complex which leads to the activation of catalytic centers. Despite the lack of evidence suggesting an exact mechanism for the cleavage of DNA by HindIII, site-mutagenesis analysis coupled with more detailed studies of metal ion-mediated catalysis in EcoRV have led to the following proposed catalytic mechanism. It has been suggested that during the hydrolysis of DNA by EcoRV the catalytic residue Lys-92 stabilizes and orients the attacking water nucleophile, while the carboxylate of Asp-90 stabilizes the leaving hydroxide anion through to coordination of Mg2
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What is the cutting site of BamHI and HindIII?
BamHI cuts at the sequence 5'-G^GATCC-3', creating sticky ends with a 5'-overhang. HindIII cuts at the sequence 5'-A^AGCTT-3', creating sticky ends with a 5'-overhang as well.
EcoRI and HindIII are What?
EcoRI and HindIII are restriction enzymes commonly used in molecular biology to cut DNA at specific sequences. They are named after the bacteria species where they were first isolated, Escherichia coli RY13 and Haemophilus influenzae Rd, respectively.
What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?
Two different restriction enzymes commonly used to cut the pUC19 plasmid are EcoRI and PstI. For cutting the lux gene DNA, the restriction enzymes commonly used are NcoI and HindIII.
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
What are some common design primers with restriction sites used in molecular biology experiments?
Common design primers with restriction sites used in molecular biology experiments include those for enzymes like EcoRI, BamHI, HindIII, and XhoI. These primers are designed to have specific sequences that match the recognition sites of these restriction enzymes, allowing for targeted DNA cleavage and manipulation.
How many sites are in pBR322 for HindIII?
pBR322 has one HindIII restriction site. This means that the HindIII enzyme can cut the pBR322 plasmid at a specific location, resulting in two fragments. The presence of this site is often utilized in molecular cloning and recombinant DNA technology.
What is the difference between Puc18 and Puc19?
The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.pUC18: LacZ HindIII PaeI ..... SacI EcoRIpUC19: Lacz EcoRI SacI ..... PaeI HindIII
What is the cutting site of BamHI and HindIII?
BamHI cuts at the sequence 5'-G^GATCC-3', creating sticky ends with a 5'-overhang. HindIII cuts at the sequence 5'-A^AGCTT-3', creating sticky ends with a 5'-overhang as well.
EcoRI and HindIII are What?
EcoRI and HindIII are restriction enzymes commonly used in molecular biology to cut DNA at specific sequences. They are named after the bacteria species where they were first isolated, Escherichia coli RY13 and Haemophilus influenzae Rd, respectively.
What group of organisms provides a source of restriction enzymes for genetic engineering?
Bacteria. The enzymes are typically named after the bacteria also. For example EcoRI comes from E. coli and HindIII comes from H. influenzae.
What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?
Two different restriction enzymes commonly used to cut the pUC19 plasmid are EcoRI and PstI. For cutting the lux gene DNA, the restriction enzymes commonly used are NcoI and HindIII.
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
What are some common design primers with restriction sites used in molecular biology experiments?
Common design primers with restriction sites used in molecular biology experiments include those for enzymes like EcoRI, BamHI, HindIII, and XhoI. These primers are designed to have specific sequences that match the recognition sites of these restriction enzymes, allowing for targeted DNA cleavage and manipulation.
A formula prewritten by excel is called?
Function
What is the parent function for the exponential function?
The parent function of the exponential function is ax
What function tests a specific condition in Excel?
The IF function is the main function to do it and you can also use other logical functions, like the AND function, the OR function or the NOT function.
What function in Microsoft Excel 2003 to write the amount in word?
Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.Such a function does not exist for English. A function call BAHTTEXT will do it for the Thai language.
