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What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?
Two different restriction enzymes commonly used to cut the pUC19 plasmid are EcoRI and PstI. For cutting the lux gene DNA, the restriction enzymes commonly used are NcoI and HindIII.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
What are some common design primers with restriction sites used in molecular biology experiments?
Common design primers with restriction sites used in molecular biology experiments include those for enzymes like EcoRI, BamHI, HindIII, and XhoI. These primers are designed to have specific sequences that match the recognition sites of these restriction enzymes, allowing for targeted DNA cleavage and manipulation.
What is the cutting site of BamHI and HindIII?
BamHI cuts at the sequence 5'-G^GATCC-3', creating sticky ends with a 5'-overhang. HindIII cuts at the sequence 5'-A^AGCTT-3', creating sticky ends with a 5'-overhang as well.
What is the difference between Puc18 and Puc19?
The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.pUC18: LacZ HindIII PaeI ..... SacI EcoRIpUC19: Lacz EcoRI SacI ..... PaeI HindIII
What group of organisms provides a source of restriction enzymes for genetic engineering?
Bacteria. The enzymes are typically named after the bacteria also. For example EcoRI comes from E. coli and HindIII comes from H. influenzae.
What is the role of ECORI?
It splices the genome or plasmid in a specific location (EcoRI).
What is the role of EcoRI enzyme?
It splices the genome or plasmid in a specific location (EcoRI).
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?
Two different restriction enzymes commonly used to cut the pUC19 plasmid are EcoRI and PstI. For cutting the lux gene DNA, the restriction enzymes commonly used are NcoI and HindIII.
How many sites are in pBR322 for HindIII?
pBR322 has one HindIII restriction site. This means that the HindIII enzyme can cut the pBR322 plasmid at a specific location, resulting in two fragments. The presence of this site is often utilized in molecular cloning and recombinant DNA technology.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
What are some common design primers with restriction sites used in molecular biology experiments?
Common design primers with restriction sites used in molecular biology experiments include those for enzymes like EcoRI, BamHI, HindIII, and XhoI. These primers are designed to have specific sequences that match the recognition sites of these restriction enzymes, allowing for targeted DNA cleavage and manipulation.
What is a degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA?
A restriction enzyme is a degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA. These enzymes are often used in biotechnology to cut DNA at specific sites for genetic engineering purposes.
What is the cutting site of BamHI and HindIII?
BamHI cuts at the sequence 5'-G^GATCC-3', creating sticky ends with a 5'-overhang. HindIII cuts at the sequence 5'-A^AGCTT-3', creating sticky ends with a 5'-overhang as well.
What is the EcoRI cuts DNA everywhere the base pattern?
EcoRI is a restriction enzyme that recognizes and cuts DNA at a specific base pattern: the palindromic sequence GAATTC. It cleaves between the G and the A on each strand, resulting in "sticky ends" that can facilitate the ligation of DNA fragments. This characteristic makes EcoRI a valuable tool in molecular biology for cloning and genetic engineering.
