0
It splices the genome or plasmid in a specific location (EcoRI).
What else can I help you with?
What is the role of EcoRI enzyme?
It splices the genome or plasmid in a specific location (EcoRI).
What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
Does pBR322 contain EcoR1 sites?
Yes, pBR322 contains EcoRI restriction sites. Specifically, there are two EcoRI sites located within the plasmid's multiple cloning site (MCS), allowing for the insertion of foreign DNA. This feature makes pBR322 a useful vector for cloning purposes in molecular biology.
EcoRI and HindIII are What?
EcoRI and HindIII are restriction enzymes commonly used in molecular biology to cut DNA at specific sequences. They are named after the bacteria species where they were first isolated, Escherichia coli RY13 and Haemophilus influenzae Rd, respectively.
If you took a linear piece of DNA and cut it with the restriction enzyme EcoRI and it had three restriction sites for EcoRI, how many fragments would you produce What if you had a circular piece of DNA?
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
What group of organisms provides a source of restriction enzymes for genetic engineering?
Bacteria. The enzymes are typically named after the bacteria also. For example EcoRI comes from E. coli and HindIII comes from H. influenzae.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
What is the difference between Puc18 and Puc19?
The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.pUC18: LacZ HindIII PaeI ..... SacI EcoRIpUC19: Lacz EcoRI SacI ..... PaeI HindIII
What are the two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA?
Two different restriction enzymes commonly used to cut the pUC19 plasmid are EcoRI and PstI. For cutting the lux gene DNA, the restriction enzymes commonly used are NcoI and HindIII.
Why is it important to inactivate the shrimp alkaline phosphatase before ligating your EcoRI cut pBluescript plasmid to a foreign DNA fragment?
because you need the phosphate of the foreign DNA fragment for ligation. If not inactivated before you add the fragment, SAP will imediately remove the phosphate form your fregment too leaving you with two dephosphorylated fragments that are not easy to ligate..
