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What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
What is the role of ECORI?
It splices the genome or plasmid in a specific location (EcoRI).
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
How can an enzyme be a catalyst?
An enzyme has the role of a catalyst but in biochemistry.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
What is the role of enzyme in cells?
enzyme sped up the chemical reaction
What is a degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA?
A restriction enzyme is a degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA. These enzymes are often used in biotechnology to cut DNA at specific sites for genetic engineering purposes.
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
What enzyme plays a role in replication?
the make food
Which enzyme would cut the human DNA shown in Part A on both sides of the vgp gene but not inside the gene?
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
How does adding an acid to an enzyme reaction effect the enzyme?
it alters the pH of the enzyme denaturing it leaving it unable to carry out it's role effectively or at all
