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What is the specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site?
The specific DNA sequence recognized by the EcoRI restriction enzyme, known as the EcoRI cut site, is 5'-GAATTC-3'.
Where does the restriction enzyme EcoRI cut in a DNA sequence?
The restriction enzyme EcoRI cuts DNA at a specific sequence of bases, which is GAATTC.
What is the role of ECORI?
It splices the genome or plasmid in a specific location (EcoRI).
Which restriction enzyme did you use to cut the DNA?
The restriction enzyme used to cut the DNA was EcoRI.
What is the EcoRI cuts DNA everywhere the base pattern?
EcoRI is a restriction enzyme that recognizes and cuts DNA at a specific base pattern: the palindromic sequence GAATTC. It cleaves between the G and the A on each strand, resulting in "sticky ends" that can facilitate the ligation of DNA fragments. This characteristic makes EcoRI a valuable tool in molecular biology for cloning and genetic engineering.
Why are recombinant plasmids cut with ecoRI?
Recombinant plasmids are cut with the restriction enzyme EcoRI because it recognizes a specific palindromic DNA sequence (GAATTC) and cleaves it at precise locations, creating compatible sticky ends. This allows for the insertion of foreign DNA fragments into the plasmid, facilitating cloning and gene expression. EcoRI's ability to produce cohesive ends promotes efficient ligation of the desired DNA insert, ensuring successful recombinant DNA formation.
How can an enzyme be a catalyst?
An enzyme has the role of a catalyst but in biochemistry.
What is Example of restriction enzyme?
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:5'-- GAATTC --3'3'-- CTTAAG --5'After this DNA sequence is cut, it might look something like this:5'-- G AATTC --3'3'-- C TTAAG --5'
What is a degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA?
A restriction enzyme is a degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA. These enzymes are often used in biotechnology to cut DNA at specific sites for genetic engineering purposes.
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
What is the sequence of Eco R1?
EcoRI is a restriction enzyme that recognizes and cuts DNA at the specific palindromic sequence GAATTC. It cleaves between the G and the A on each strand, resulting in sticky ends that can be used for DNA cloning and other molecular biology applications. The recognition sequence is crucial for the enzyme's function in genetic engineering.
What is the role of enzyme in cells?
enzyme sped up the chemical reaction
