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Restriction enzymes are not typically used in PCR. PCR relies on DNA polymerase to amplify specific DNA sequences, while restriction enzymes are used to cut DNA at specific recognition sites for other applications, such as cloning.

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6mo ago

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Which of the following cuts DNA molecules at specific locations?

Restriction enzymes are the molecular scissors that cut DNA molecules at specific locations by recognizing and binding to specific DNA sequences. This process is essential in genetic engineering and molecular biology techniques such as gene cloning and PCR.


Why are restriction enzymes necessary when trying to analyze DNA?

Restriction enzymes are necessary because they can cut DNA at specific recognition sites, enabling the manipulation of DNA fragments for analysis. They are used to generate predictable DNA fragments and are crucial for techniques like PCR, genetic engineering, and gene cloning. By cutting DNA at specific locations, restriction enzymes allow for targeted analysis and manipulation of DNA sequences.


Which pair of enzymes is necessary to make recombinant DNA?

Restriction enzymes and DNA ligase are necessary to make recombinant DNA. Restriction enzymes are used to cut the DNA at specific sequences, while DNA ligase is used to join together pieces of DNA from different sources.


Which enzyme opens DNA molecules?

Restriction Enzymes


What are the uses of restriction enzymes in biotechnology?

Restriction enzymes or endonucleases are like cutting enzymes fro DNA These are used to cut nucleotides at particular sites These have imp use in gene cloning,gene mapping,gene sequencing then applied techniques such as southern blotting These are extracted from bacteria's


What enzyme do scientists use to cut genes out of strands if DNA?

restriction enzymes


Which enzyme is used to produce RFLPs?

Restriction enzymes.


Where do restriction enzymes originate from?

Restriction enzymes originate from bacteria.


What is the purpose of restriction enzymes?

Restriction enzymes are used to cut DNA at specific sequences, allowing for manipulation of DNA in genetic engineering techniques like cloning, PCR, and DNA sequencing. They are essential tools in molecular biology for creating recombinant DNA molecules.


What is used to cut the DNA chain so that new genes may be inserted?

Enzymes called restriction endonucleases are used to cut the DNA chain at specific recognition sites. These enzymes recognize and cleave the DNA at particular sequences, allowing new genes to be inserted at the site of the cut.


Can Restriction Enzymes be used on all DNA?

Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis


What are used to cleave DNA into fragments?

Restriction enzymes. Babe