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If you used distilled water instead of alcohol in a gram staining procedure what would be the colour of gram positive and what of gram negative bacteria?
Wiki User
∙ 14y ago
If we use distill water in gram staining instead of alcohol then both gram +ve and gram -ve will appear blue as distilled water is not able to remove crystal violet from cell wall and so when we counter stain the cell with safranin it will give no effect of that and cell show only blue colour
Wiki User
∙ 14y ago
Wiki User
∙ 14y agoThe same color, as the gram negative bacteria would not have enough stain washed away to assume their lighter shade. Remember, the thickness and construction of the peptidoglycan that forms the walls of the two types of bacteria varies significantly. Gram positive bacteria have the single, thick wall. Gram negative have a thinner and double wall that does not retain the stain as well.
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