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What does reverse transcriptase use from DNA?
Reverse transcriptase use mRNA to form DNA. mRNA
What does a eukaryotic cell use to make reverse transcriptase?
A eukaryotic cell uses its own enzymes to make reverse transcriptase.
Retroviruses such as HIV contains what enzyme that enables DNA to be synthesized from rna?
Retroviruses such as HIV contain the enzyme called reverse transcriptase, which enables the synthesis of DNA from RNA. This DNA integrates into the host cell genome, allowing the virus to replicate and persist in the host.
What happens when RNA is extracted from a virus is injected into a host cell?
Without the viral enzyme reverse transcriptase probable nothing. RNA viruses are retroviruses because they nee reverse tanscriptase to make a DNA copy from the RNA that is inserted into the cell. Generally to form a provirus. Now if you injected DNA from a regular virus then it would be as if the virus did it itself.
What is is called when RNA copies to DNA?
When RNA's base sequence is used to determine the base sequence of a new strand of DNA, that is called reverse transcription.This is because the process is the reverse of transcription, which involves copying the base sequence of DNA to form RNA, including messenger RNA (mRNA).
What is thepurpose of using reverse transcriptase?
To make DNA from mRNA
What does reverse transcriptase use from DNA?
Reverse transcriptase use mRNA to form DNA. mRNA
What enzyme do you need to get a copy of DNA from mRNA?
The enzyme needed to make a DNA copy of RNA (including mRNA) is called reverse transcriptase. This enzyme is capable of synthesizing a complementary DNA strand from an RNA template, which is the first step in generating a cDNA (complementary DNA) library.
If this RNA strand is used to make a DNA strand what process will have occurred?
If the RNA strand is used to make a DNA strand, the process that has occurred is called reverse transcription. This process is carried out by the enzyme reverse transcriptase, which synthesizes complementary DNA (cDNA) from the RNA template. Reverse transcription is commonly associated with retroviruses, where the viral RNA is converted into DNA for integration into the host genome.
Why are using reverse transcriptase necessary for finding a way to make artificial insulin?
Reverse transcriptase is necessary for making artificial insulin because it converts RNA into complementary DNA (cDNA). Since the insulin gene is often expressed in the form of mRNA in cells, reverse transcriptase enables researchers to create a stable, DNA version of this gene. This cDNA can then be inserted into plasmids for cloning and expression in bacteria or yeast, allowing for the production of recombinant insulin. This process is crucial for generating large quantities of insulin for therapeutic use.
How do retrovirsus produce DNA from and rna blueprint?
Simply by taking the loose nucleotides of DNA in the cell's cytosol and using the enzyme reverse transcriptase to make a DNA from the RNA template. Retroviruses are carrying their own reverse transcriptase to do this very thing.
What does a eukaryotic cell use to make reverse transcriptase?
A eukaryotic cell uses its own enzymes to make reverse transcriptase.
What is the role of reverse transcriptase?
Reverse transcriptase is the viral enzyme responsible for making a DNA copy of the RNA genome from a retrovirus. As such, it is technically known as an RNA-dependent DNA polymerase. This is opposite to the usual flow of genetic information from DNA to RNA to protein. This allows a virus with an RNA genome to make copies of itself that can be integrated into the DNA of its host.
What is Replication of an RNA virus?
You might be referring to the reverse-transcription that many retroviruses use (a virus with an RNA that turns into DNA). By using the enzyme reverse transcriptase, viruses like HIV can make a DNA from RNA template.
How do you make a complementary RNA?
cDNA of the gene/mRNA for which you want to make complementary RNA is cloned into special plasmid vectors that have promoters at either ends of the multiple cloning sites (MCS). Using RNA polymerase and nucleotides you can now synthesize RNA complementary to the original mRNA. This is called complementary RNA (cRNA).
Retroviruses such as HIV contains what enzyme that enables DNA to be synthesized from rna?
Retroviruses such as HIV contain the enzyme called reverse transcriptase, which enables the synthesis of DNA from RNA. This DNA integrates into the host cell genome, allowing the virus to replicate and persist in the host.
Is it true or false that hiv uses reverse transcriptase to make ds RNA copies of its DNA genome?
False. HIV uses reverse transcriptase to make single-stranded RNA copies of its RNA genome.
