There are a huge number of steps involved in this process, but briefly it goes like this:
# Transcription factors bind to DNA and promote binding of RNA polymerase # RNA polymerase binds in complex with transcription factors # Transcription is initiated and RNA polymerase uses free NTPs, complementary to the antisense strand of DNA to create an RNA copy of the DNA.
# The mRNA leaves the nucleus - some modification may take place
# Translation factors bind mRNA # Ribosome binds to mRNA # tRNA brings amino acids to ribosome # Ribosome begins translation at methionine start codon - matching anticodon on tRNA to triplet codons on mRNA to pair correct amino acid to the mRNA code # Ribosome stops translation at stop codon # Ribosome/mRNA/tripeptide complex will dissociate
amino acid
one amino acid in the sequence would change
3 nucleotides
mRNA has the codon sequence that exactly correspond to the amino acid sequence. The DNA has two strands. Though one of the strands (coding strand) carries the same information as on the mRNA, it contains unspliced introns that will mess up the amino acid sequence.
A linear stretch of DNA that specifies the sequence of amino acids in a polypeptide is called a gene. The primary function of DNA ligase is to seal new short stretches of nucleotides into one continuous strand.
The addition or deletion of a nucleotide can lead to a Frameshift mutation. The Frameshift mutation causes a "shift" in the reading frame of the codons in the mRNA. This may lead to the change in the amino acid sequence at protein translation.
one amino acid in the sequence would change
Serine, Methionine , Leucine.
Serine, Isoleucine. Last codon is incomplete.
The template strand is mutated to 3'-TACTTGTCCAATATC-5'.
3 nucleotides
ASN, ALA, PHE, ARG.
mRNA has the codon sequence that exactly correspond to the amino acid sequence. The DNA has two strands. Though one of the strands (coding strand) carries the same information as on the mRNA, it contains unspliced introns that will mess up the amino acid sequence.
The sequence of amino acids (forming a protein) that result from the mRNA strand CAG-AAG-UUC-CUC-UCG-C would be: Glutamine-Threonine-Phenylalanine-Leucine-Serine Each codon must be three bases long - therefore the end of this mRNA sequence 'C' cannot code for an amino acid. There would also need to be a stop codon at the end to complete translation.
A linear stretch of DNA that specifies the sequence of amino acids in a polypeptide is called a gene. The primary function of DNA ligase is to seal new short stretches of nucleotides into one continuous strand.
The addition or deletion of a nucleotide can lead to a Frameshift mutation. The Frameshift mutation causes a "shift" in the reading frame of the codons in the mRNA. This may lead to the change in the amino acid sequence at protein translation.
Insertions generally are more harmful. An insertion causes a "frameshift" to occur, thus changing the entire amino acid sequence of the entire strand. Substitutions only change one base, which can sometimes not mess up the amino acid sequence at all.
A strand of DNA